SubtiBank SubtiBank
yfnH
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yfnH

similar to glucose-1-phosphate cytidylyltransferase
locus
BSU_07270
pI
5.49
mw
28.52 kDa
protein length
254 aa Sequence Blast
gene length
765 bp Sequence Blast
function
unknown
product
unknown
essential
no
ec
2.7.7.33
synonyms

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]
  • Gene

    Coordinates
    798,469 → 799,233

    The protein

    Catalyzed reaction/ biological activity

  • α-D-glucose 1-phosphate + CTP + H+ --> CDP-D-glucose + diphosphate (according to UniProt)
  • Protein family

  • cytidylyltransferase family (with [protein|CF9633F769263C6D16EE778F6BD2ECF1995F512A|IspD] and [protein|8620DA76A1953D7ACDFA7E46ED81EF1A0D97999C|TagD], according to UniProt)
  • glucose-1-phosphate cytidylyltransferase family (single member, according to UniProt)
  • Structure

  • [PDB|1TZF] (from ''Salmonella Typhi'', 43% identity)
  • Expression and Regulation

    Operons

    genes
    [gene|B25AE6979AF12628780C4489FFED966C6B31968A|yfnH]-[gene|9836E90BD443F5269A43E5C69FCF2CCB22EDF65B|yfnG]-[gene|4148A3F8E256D9B1D667C28428D867B5B6B5F3CE|yfnF]-[gene|E3481379D3FEC02C35CB4BE17A5246A208C5C86D|yfnE]-[gene|1D0286ECDA32FB21737170B54A57D3FE482C6422|yfnD]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15383836], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]
  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, internal promoter in [protein|E3481379D3FEC02C35CB4BE17A5246A208C5C86D|YfnE] open reading frame [Pubmed|15383836], see [http://dbtbs.hgc.jp/COG/prom/yfnHGFED.html DBTBS] for details, in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]
  • regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE]: activation, [Pubmed|26577401], in [regulon|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE regulon]
  • regulation

  • expressed during sporulation ([protein|search|SigK]) [Pubmed|15383836]
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    Biological materials

    Mutant

  • MGNA-C227 (yfnH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2225 NBRP B. subtilis, Japan]
  • BKE07270 (Δ[gene|B25AE6979AF12628780C4489FFED966C6B31968A|yfnH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCTACCCCTCATCTCT, downstream forward: _UP4_GTATGGAAGGTATGGTGAAT
  • BKK07270 (Δ[gene|B25AE6979AF12628780C4489FFED966C6B31968A|yfnH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCTACCCCTCATCTCT, downstream forward: _UP4_GTATGGAAGGTATGGTGAAT
  • References

  • 15383836,26577401