SubtiBank SubtiBank
yphC [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]
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yphC [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]

GTPase essential for ribosome 50S subunit assembly
locus
BSU22840
pI
5.00
mw
48.00 kDa
function
ribosome assembly
product
GTPase
essential
yes
synonyms
engA

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.6|Ribosome assembly]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.3|GTP-binding proteins]
  • [SW|Categories] containing this gene/protein

  • [SW|translation], [SW|essential genes], [SW|GTP-binding proteins]
  • Gene

    Coordinates on the chromosome (coding sequence)
    2,390,206 -> 2,391,516

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • binds and hydrolyzes GTP and readily exchanges GDP for GTP
  • participates in the maturation of the central protuberance, GTPase associated region and key RNA helices in the A, P and E functional sites of the 50S subunit [Pubmed|27484475]
  • Protein family

  • EngA subfamily (according to Swiss-Prot) Era/Obg family
  • Paralogous protein(s)

  • [protein|search|Era], [protein|search|Obg], [protein|search|YsxC], [protein|search|RbgA], [protein|search|YqeH], [protein|search|ThdF], [protein|search|YyaF], [protein|search|YnbA]
  • Structure

  • [PDB|2HJG] (complex with GDP)
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • Biological materials

    Expression vector

  • for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP846, available in [SW|Jörg Stülke]'s lab
  • Labs working on this gene/protein

  • [SW|Naotake Ogasawara], Nara, Japan
  • References

    Reviews

  • 19575570,21885683
  • Original publications

  • 12427945,16997968,16894162,16682769,23056455,27484475