yphC [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]

GTPase essential for ribosome 50S subunit assembly

locus: BSU22840

pI: 5.00

mw: 48.00 kDa

function: ribosome assembly

product: GTPase

essential: yes

synonyms: engA

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.6|Ribosome assembly]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.3|GTP-binding proteins]

[SW|Categories] containing this gene/protein

• [SW|translation], [SW|essential genes], [SW|GTP-binding proteins]

Gene

Coordinates on the chromosome (coding sequence): 2,390,206 -> 2,391,516

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• binds and hydrolyzes GTP and readily exchanges GDP for GTP
• participates in the maturation of the central protuberance, GTPase associated region and key RNA helices in the A, P and E functional sites of the 50S subunit [Pubmed|27484475]

Protein family

• EngA subfamily (according to Swiss-Prot) Era/Obg family

Paralogous protein(s)

• [protein|search|Era], [protein|search|Obg], [protein|search|YsxC], [protein|search|RbgA], [protein|search|YqeH], [protein|search|ThdF], [protein|search|YyaF], [protein|search|YnbA]

Structure

• [PDB|2HJG] (complex with GDP)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Biological materials

Expression vector

• for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP846, available in [SW|Jörg Stülke]'s lab

Labs working on this gene/protein

• [SW|Naotake Ogasawara], Nara, Japan

References

Reviews

• 19575570,21885683

Original publications

• 12427945,16997968,16894162,16682769,23056455,27484475