SubtiBank SubtiBank
yphC
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yphC

GTPase essential for ribosome 50S subunit assembly
locus
BSU_22840
pI
5.25
mw
48.61 kDa
protein length
436 aa Sequence Blast
gene length
1311 bp Sequence Blast
function
ribosome assembly
product
GTPase
essential
yes
synonyms
engA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.6|Ribosome assembly]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.3|GTP-binding proteins]

    • Gene

    Coordinates
    2,390,206 → 2,391,516

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • binds and hydrolyzes GTP and readily exchanges GDP for GTP
  • participates in the maturation of the central protuberance, GTPase associated region and key RNA helices in the A, P and E functional sites of the 50S subunit [Pubmed|27484475]

    • Protein family

  • [SW|TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily] (according to UniProt)

    • Paralogous protein(s)

  • [protein|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|YyaF], [protein|CB68B3393CA7862C45C624AF7B1ADD188243A01F|Era], [protein|DF1A043F3D9817D9479B8C707F4ACEEF2BF2862C|YsxC], [protein|54CD247926F7FABE4ACE85EB7021B62500B47260|RbgA], [protein|66A6EC4F6CB91EE741BB9D7646913FF3EE602BD7|ThdF], [protein|84E7D672DB40B114DC4D96A4D8834958B47401A7|Obg], [protein|959C0ED7B9DA50F0EADCF1565405B878D36DA206|YqeH], [protein|9F6B78C1932FB56F7F71430DB16BC862A312407B|YnbA]

    • [SW|Domains]

  • 2 EngA-type G domains (aa 4-167, aa 176-351) (according to UniProt)
  • KH-like domain (aa 352-436) (according to UniProt)

    • Structure

  • [PDB|2HJG] (complex with GDP) [pubmed|16894162]
  • [PDB|5M7H] (complex with GMPPNP and PO4)

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|B2270316642C66BC2DA86EBDCD1BF6A33CC6A1DC|yphC]-[gene|8440C1DBE485BB9EF2904FAA5D709A574C3819A4|gpsA]
    description
    [pubmed|22383849]
    view in new tab

    genes
    [gene|5DA8B27AC6A0E0B4115512BD948C31964D387A8E|yphA]-[gene|448375DF74EB0482A6CD1ACFE04D117C1319E2B2|seaA]-[gene|910109496E4A41EC892996DEEB81971FCBD9CB05|ypzH]-[gene|B2270316642C66BC2DA86EBDCD1BF6A33CC6A1DC|yphC]-[gene|8440C1DBE485BB9EF2904FAA5D709A574C3819A4|gpsA]
    description
    [pubmed|22383849]
    view in new tab

    additional information

  • the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]

    • Biological materials

    Mutant

  • MGNA-A402 (yphC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/402 NBRP B. subtilis, Japan]

    • Expression vectors

  • for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP846, available in [SW|Jörg Stülke]'s lab

    • labs

  • [SW|Naotake Ogasawara], Nara, Japan

    • References

    Reviews

  • 19575570,21885683

    • Original publications

  • 12427945,16997968,16894162,16682769,23056455,27484475,26883633