SubtiBank SubtiBank
gyrA
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

gyrA

DNA gyrase (subunit A)
locus
BSU00070
pI
5.22
mw
91.91 kDa
protein length
821 aa Sequence Blast
gene length
2463 bp Sequence Blast
function
DNA supercoiling, initation of replication cycle and DNA elongation
product
DNA gyrase (subunit A)
essential
yes
ec
5.99.1.3
synonyms
nalA

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.3|DNA condensation/ segregation]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    6,994 → 9,459

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP-dependent breakage, passage and rejoining of double-stranded DNA (according to Swiss-Prot)
  • Protein family

  • topoisomerase gyrA/parC subunit family (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|9075DD0CC0B8554F0379732453665F43C70EFC26|ParC]
  • Structure

  • [PDB|4DDQ] (DNA gyrase A-subunit lacking the C-terminal DNA-wrapping domains) [Pubmed|23602808]
  • [SW|Localization]

  • Nucleoid (Homogeneous) [Pubmed|16479537]
  • nucleoid-associated in actively growing cells [Pubmed|9539793]
  • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]
  • Expression and Regulation

    Operons

    genes
    [gene|FB1C21A94697E5B9D29C0F3B9C9F36E57BCBD237|yaaA]-[gene|EBE03AEC7EC594A3115A7A72194BDFF300AA0BFA|recF]-[gene|CBC76FB453569550F14FE5015C05D45985C04979|remB]-[gene|AA42910B71348EFEA4165CFFCEF1E497897B9DDC|gyrB]-[gene|B207C7328177924B16F1C606F667BEADF12CA069|gyrA]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2987848], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
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    Labs working on this gene/protein

  • [SW|Dagmar Klostermeier], University of Münster, Germany, [http://www.biozentrum.unibas.ch/klostermeier/index.html homepage]
  • References

  • 16479537,17981983,9539793,2987848,19666507,17320901,23475963,23602808,20870749,24563461,27557712,27793284,12767818