ydfD

similar to transcriptional regulator ([SW|MocR/ GabR family])

locus: BSU_05370

pI: 9.10

mw: 54.07 kDa

protein length: 482 aa

gene length: 1449 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.6|Transcription factor/ other/ based on similarity]

Gene

Coordinates: 583,589 → 585,037

The protein

Protein family

• [SW|class-I pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)
• [SW|MocR/ GabR family] [Pubmed|22020104]

Paralogous protein(s)

• [protein|6A3F08CD939CCDEC1F784D658B815A6D0E1AAC3D|YisV]

[SW|Domains]

• N-terminal DNA-binding helix-turn-helix motif; C-terminal domain is homologous to PLP-binding large domain of aminotransferases.
• [SW|HTH gntR-type domain] (aa 12-80) (according to UniProt)

[SW|Cofactors]

• PLP

Structure

• [PDB|1WST] (aminotransferase from Thermococcus profundus, 30% identity)

Biological materials

Mutant

• MGNA-C144 (ydfD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2142 NBRP B. subtilis, Japan]
• BKE05370 (Δ[gene|B1A95EAE40AFA40BF12EE9B41287921417A39562|ydfD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGAATCGGTCTCCTTAA, downstream forward: _UP4_TAACTTTTATATCCTTAAAT
• BKK05370 (Δ[gene|B1A95EAE40AFA40BF12EE9B41287921417A39562|ydfD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGAATCGGTCTCCTTAA, downstream forward: _UP4_TAACTTTTATATCCTTAAAT

References

• 11756427,22020104