katA [Wed Jan 20 2016 15:56:23 GMT+0100 (CET)]

main vegetative catalase 1

locus: BSU08820

pI: 6.00

mw: 54.00 kDa

function: detoxification (degradation) of hydrogen peroxide

product: vegetative catalase

essential: no

synonyms: kat-19

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

[SW|Categories] containing this gene/protein

• [SW|resistance against oxidative and electrophile stress], [SW|phosphoproteins]

This gene is a member of the following [SW|regulons]

• [SW|PerR regulon]

Gene

Coordinates on the chromosome (coding sequence): 959,535 -> 960,986

Phenotypes of a mutant

• increased sensitivity to oxidative stress [Pubmed|22174384]
• increased susceptibility to Cr(VI) due to the accumulation of oxidative DNA damage [Pubmed|24973075]

The protein

Catalyzed reaction/ biological activity

• 2 H₂O₂ = O₂ 2 H₂O (according to Swiss-Prot)

Protein family

• catalase family (according to Swiss-Prot)

Modification

• phosphorylated on Arg-366 [Pubmed|22517742]

[SW|Cofactors]

• contains an iron-sulfur cluster, heme

Structure

• [PDB|1SI8] (enzyme from Enterococcus faecalis, 68% identity)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operon

• ''katA'' [Pubmed|22174384]

Regulation

• induced in the presence of hydrogen peroxide and paraquat ([protein|search|PerR]) [Pubmed|11532148,22174384]

Regulatory mechanism

• [protein|search|PerR]: transcription repression [Pubmed|11532148]

References

• 9393707,7667267,8931328,8932315,11532148,22174384,22194458,22517742,24973075