katA [2017-04-24 15:34:12]

main vegetative catalase 1

locus: BSU08820

pI: 6.15

mw: 54.57 kDa

protein length: 483 aa

gene length: 1449 bp

function: detoxification (degradation) of hydrogen peroxide

product: vegetative catalase

essential: no

ec: 1.11.1.6

synonyms: kat-19

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

This gene is a member of the following [SW|regulons]

• [SW|PerR regulon]

Gene

Coordinates: 959,535 → 960,986

Phenotypes of a mutant

• increased sensitivity to oxidative stress [Pubmed|22174384]
• increased susceptibility to Cr(VI) due to the accumulation of oxidative DNA damage [Pubmed|24973075]

The protein

Catalyzed reaction/ biological activity

• 2 H₂O₂ = O₂ + 2 H₂O (according to Swiss-Prot)

Protein family

• catalase family (according to Swiss-Prot)

Modification

• phosphorylated on Arg-366 [Pubmed|22517742]

[SW|Cofactors]

• contains an iron-sulfur cluster, heme

Structure

• [PDB|1SI8] (enzyme from Enterococcus faecalis, 68% identity)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|B0A1AB6E920E6CBEE45115297599AF9A80972E38|katA]
  description: [Pubmed|22174384]

  regulatory mechanism

  • [protein|00BCAAE16576DC5426A652580C69A570FC7A1C2C|PerR]: repression, [Pubmed|11532148], in [regulon|00BCAAE16576DC5426A652580C69A570FC7A1C2C|PerR regulon]

  regulation

  • induced in the presence of hydrogen peroxide and paraquat ([protein|search|PerR]) [Pubmed|11532148,22174384]

References

• 9393707,7667267,8931328,8932315,11532148,22174384,22194458,22517742,24973075