yvcT [2019-02-08 17:29:24]

similar to hydroxypyruvate reductase

locus: BSU34680

pI: 5.09

mw: 36.46 kDa

protein length: 325 aa

gene length: 978 bp

function: unknown

product: unknown

essential: no

ec: 1.1.1.215

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

Gene

Coordinates: 3,562,566 → 3,563,543

The protein

Catalyzed reaction/ biological activity

• D-gluconate + NADP⁺ = 2-dehydro-D-gluconate + NADPH (according to Swiss-Prot)

Protein family

• D-isomer specific 2-hydroxyacid dehydrogenase family (according to Swiss-Prot)

Structure

• [PDB|5AOV] (from Pyrococcus furiosus, 46% identity)

Additional information

• The gene is annotated in KEGG as gluconate 2-dehydrogenase EC 1.1.1.215. The gene is marked “similar to glycerate dehydrogenase” in MetaCyc and marked as probable EC 1.1.1.215 in Swiss-Prot. No literature/experimental evidence supporting the annotation is available. [Pubmed|19935659]

Expression and Regulation

Operons

• genes: [gene|B0145F4E13F004ECC773E8758B5844669F4C74D7|yvcT]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B633 (yvcT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1632 NBRP B. subtilis, Japan]
• BKE34680 (Δ[gene|B0145F4E13F004ECC773E8758B5844669F4C74D7|yvcT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGGCTCCCCCTTTATGGC, downstream forward: _UP4_TAAATCAAAAATCCGGTCTG
• BKK34680 (Δ[gene|B0145F4E13F004ECC773E8758B5844669F4C74D7|yvcT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGGCTCCCCCTTTATGGC, downstream forward: _UP4_TAAATCAAAAATCCGGTCTG