SAVE THE DATE!
21st International Conference on Bacilli and Gram-Positive Bacteria. Prague, Czech Republic, June 7-11, 2021.
similar to hydroxypyruvate reductase
categories[category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]
3,562,566 → 3,563,543
Catalyzed reaction/ biological activityD-gluconate + NADP+ --> 2-dehydro-D-gluconate + H+ + NADPH (according to UniProt)
Protein familyD-isomer specific 2-hydroxyacid dehydrogenase family (with [protein|3769B79A180412C0FE98B3FC54BA49750BAFEDF6|YoaD] and [protein|8AD1C7C761FF8B407A973381CA135C264B995CB7|SerA], according to UniProt)
Structure[PDB|5AOV] (from Pyrococcus furiosus, 46% identity) [pubmed|26865263]
Additional informationThe gene is annotated in KEGG as gluconate 2-dehydrogenase EC 18.104.22.168. The gene is marked “similar to glycerate dehydrogenase” in MetaCyc and marked as probable EC 22.214.171.124 in Swiss-Prot. No literature/experimental evidence supporting the annotation is available. [Pubmed|19935659]
Expression and Regulation
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MutantMGNA-B633 (yvcT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1632 NBRP B. subtilis, Japan]BKE34680 (Δ[gene|B0145F4E13F004ECC773E8758B5844669F4C74D7|yvcT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGGCTCCCCCTTTATGGC, downstream forward: _UP4_TAAATCAAAAATCCGGTCTGBKK34680 (Δ[gene|B0145F4E13F004ECC773E8758B5844669F4C74D7|yvcT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGGCTCCCCCTTTATGGC, downstream forward: _UP4_TAAATCAAAAATCCGGTCTG