yhcV

forespore-specific sporulation protein

locus: BSU_09230

pI: 5.01

mw: 14.77 kDa

protein length: 140 aa

gene length: 423 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

Gene

Coordinates: 997,175 → 997,597

The protein

Paralogous protein(s)

• [protein|8529D83560E934709B20CCF0B72251A74EC272BB|YlbB]:

[SW|Domains]

• 2 [SW|CBS domain]s (aa 8-64, aa 72-127) (according to UniProt)

Structure

• [PDB|4FRY] (protein from ''Burkholderia ambifaria'', 30% identity) [Pubmed|23382856]

Additional information

• The gene is annotated in KEGG as an ortholog of IMP dehydrogenase EC 1.1.1.205. No EC annotation is available in Swiss-Prot/MetaSwiss-Prot/MetaCyc.literature/experimental evidence supporting the annotation is available. [Pubmed|19935659]

Expression and Regulation

additional information

• the mRNA belongs to the 46 most abundant mRNA species in dormant spores [pubmed|30782632]

Biological materials

Mutant

• MGNA-B474 (yhcV::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1473 NBRP B. subtilis, Japan]
• BKE09230 (Δ[gene|AF3ED8916EA02A560EC8D47E4B1C911E871B9AB0|yhcV]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTCAGCACCCCTTTCA, downstream forward: _UP4_TAATAAGAAAGAGCTTGCAG
• BKK09230 (Δ[gene|AF3ED8916EA02A560EC8D47E4B1C911E871B9AB0|yhcV]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTCAGCACCCCTTTCA, downstream forward: _UP4_TAATAAGAAAGAGCTTGCAG

Expression vectors

• pGP2930 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

References

• 15699190,12480901,16497325,23382856,30782632