sspD

small acid-soluble spore protein (minor alpha/beta-type SASP)

locus: BSU_13470

pI: 8.94

mw: 6.67 kDa

protein length: 64 aa

gene length: 195 bp

function: protection of spore DNA

product: small acid-soluble spore protein (minor alpha/beta-type SASP)

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.3|Small acid-soluble spore proteins]

Gene

Coordinates: 1,413,800 → 1,413,994

The protein

Protein family

• [SW|Alpha/beta-type SASP family] (according to UniProt)

Paralogous protein(s)

• [protein|80B5DAED9B4BD015D877DB4819AB25FC5F165C6D|SspA], [protein|28F47ADECD376494878AE58B395B3A2616B6B5DF|SspB], [protein|9E1B3A6289F2C94F9088FA14CE8939E208DE9FEA|SspC]

Structure

• [PDB|2Z3X] ([protein|9E1B3A6289F2C94F9088FA14CE8939E208DE9FEA|SspC]-DNA complex, 59% identity)

Expression and Regulation

Operons

• genes: [gene|AEFA4CE1588C7EC7FCB01312A172093D12B2B206|sspD]
  description: [Pubmed|3009398]

  sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|15699190], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

  regulatory mechanism

  • [protein|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|SpoVT]: activation, [Pubmed|8755877], in [regulon|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|SpoVT regulon]

  regulation

  • expressed during sporulation in the forespore ([protein|search|SigG], [SW|SpoVT]) [Pubmed|15699190,8755877]

Biological materials

Mutant

• BKE13470 (Δ[gene|AEFA4CE1588C7EC7FCB01312A172093D12B2B206|sspD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTTCATCTCCTTTT, downstream forward: _UP4_GGCACAACTAAATAAATTCA
• BKK13470 (Δ[gene|AEFA4CE1588C7EC7FCB01312A172093D12B2B206|sspD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTTCATCTCCTTTT, downstream forward: _UP4_GGCACAACTAAATAAATTCA

References

• 3009398,15699190,8755877