SubtiBank SubtiBank
ppaC
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ppaC

inorganic pyrophosphatase
locus
BSU40550
pI
4.51
mw
33.83 kDa
protein length
309 aa Sequence Blast
gene length
927 bp Sequence Blast
function
recovery of phosphate ions from pyrophosphate
product
inorganic pyrophosphatase
essential
yes
ec
3.6.1.1
synonyms
yybQ

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.3|Phosphate metabolism]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    4,168,204 → 4,169,133

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • Diphosphate + H2O = 2 phosphate (according to Swiss-Prot)
  • Protein family

  • PPase class C family (according to Swiss-Prot)
  • [SW|Domains]

  • [SW|DHH-DHHA1 domain]
  • Modification

  • S-cysteinylation after diamide stress (C158) [Pubmed|17611193]
  • Cys158 is S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species [Pubmed|21749987] [Pubmed|21749987]
  • [SW|Cofactors]

  • Mn2+ [pubmed|17095506]
  • Structure

  • [PDB|2HAW] (complex with substrate analogue p-nitrophosphate), [PDB|1WPM] [pubmed|17095506]
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
  • Expression and Regulation

    Operons

    genes
    [gene|AE44787B3EB8EF5811B5C447BE2012EC36547B0A|ppaC]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-B837 (yybQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1836 NBRP B. subtilis, Japan]
  • References

  • 22938038,21749987,15533045,17611193,9845334,9782505,11342544,9633597,21749987,15378759,22383849,25733611,17095506