SubtiBank SubtiBank
ppaC
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

ppaC

inorganic pyrophosphatase
locus
BSU_40550
pI
4.51
mw
33.83 kDa
protein length
309 aa Sequence Blast
gene length
930 bp Sequence Blast
function
recovery of phosphate ions from pyrophosphate
product
inorganic pyrophosphatase
essential
yes
ec
3.6.1.1
synonyms
yybQ

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.3|Phosphate metabolism]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    4,168,204 4,169,133

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • diphosphate + H2O --> H+ + 2 phosphate (according to UniProt)
  • Protein family

  • PPase class C family (single member, according to UniProt)
  • [SW|Domains]

  • [SW|DHH-DHHA1 domain]
  • Modification

  • S-cysteinylation after diamide stress (C158) [Pubmed|17611193]
  • Cys158 is S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species [Pubmed|21749987] [Pubmed|21749987]
  • [SW|Cofactors]

  • Mn2+ [pubmed|17095506]
  • Structure

  • [PDB|2HAW] (complex with substrate analogue p-nitrophosphate), [PDB|1WPM] [pubmed|17095506]
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
  • Expression and Regulation

    Operons

    genes
    [gene|AE44787B3EB8EF5811B5C447BE2012EC36547B0A|ppaC]
    description
    [Pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • MGNA-B837 (yybQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1836 NBRP B. subtilis, Japan]
  • References

  • 22938038,21749987,15533045,17611193,9845334,9782505,11342544,9633597,21749987,15378759,22383849,25733611,17095506