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accB [Wed Nov 18 2015 10:17:13 GMT+0100 (CET)]
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accB [Wed Nov 18 2015 10:17:13 GMT+0100 (CET)]

acetyl-CoA carboxylase (biotin carboxyl carrier subunit)
locus
BSU24350
pI
4.00
mw
17.00 kDa
function
production of malonyl-CoA, the substrate for fatty acid biosynthesis
product
acetyl-CoA carboxylase (biotin carboxyl carrier subunit)
essential
yes
ec
6.4.1.2
synonyms
fabE, yqhW
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • [SW|Categories] containing this gene/protein

  • [SW|biosynthesis of lipids], [SW|essential genes]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,531,718 -> 2,532,197

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    [SW|Cofactors]

  • biotin [Pubmed|24816803]

    • Structure

  • [PDB|1BDO] (the protein from ''E. coli'') [Pubmed|8747466]

    • [SW|Interactions]

  • [SW|BirA]-[SW|AccB] [Pubmed|24816803]
  • ([protein|search|AccA]-[protein|search|AccD])-[protein|search|AccB]-[protein|search|AccC] (acetyl-CoA carboxylase complex)

    • Additional information

  • AccB binds to StrepTactin, and may be co-purified when purifying Strep-tagged proteins by [SW|SPINE].

    • Expression and Regulation

    Operon

  • ''[protein|search|accB]-[protein|search|accC]-[protein|search|yqhY]'' [Pubmed|7592499]

    • Biological materials

    Expression vector

  • GP1477 (chromosomal ''accB''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
  • pGP2649: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844], available in [SW|Jörg Stülke]'s lab
  • pGP2650: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab
  • pGP2652: IPTG inducible expression, purification in ''E. coli'' with C-terminal Strep-tag, in [SW|pGP574], available in [SW|Jörg Stülke]'s lab

    • GFP fusion

  • GP1493 (mls, based on [SW|pGP1080]), available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • FLAG-tag construct

  • GP1484 (''mls'', based on [SW|pGP1087]), available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 15952903,12121720,15968460

    • Original Publications

  • 7592499,8747466,24816803