SubtiBank SubtiBank
accB
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accB

acetyl-CoA carboxylase (biotin carboxyl carrier subunit)
locus
BSU24350
pI
4.39
mw
17.09 kDa
protein length
159 aa Sequence Blast
gene length
477 bp Sequence Blast
function
production of malonyl-CoA, the substrate for fatty acid biosynthesis
product
acetyl-CoA carboxylase (biotin carboxyl carrier subunit)
essential
yes
ec
6.4.1.2
synonyms
fabE,yqhW

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    2,531,718 → 2,532,197

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    [SW|Cofactors]

  • biotin [Pubmed|24816803]
  • Structure

  • [PDB|1BDO] (the protein from ''E. coli'') [Pubmed|8747466]
  • Additional information

  • AccB binds to StrepTactin, and may be co-purified when purifying Strep-tagged proteins by [SW|SPINE].
  • Expression and Regulation

    Operons

    genes
    [gene|ADD1CE87C1C5BA44FFF5499EA5E155C4C53A1C22|accB]-[gene|1DFFEB155028A5C6B4B344769EF7DAAC14983322|accC]-[gene|FACE7176B1A1F354CF27FB30E46A1140FAFB02F0|yqhY]
    description
    [Pubmed|7592499]
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    Biological materials

    Expression vector

  • GP1477 (chromosomal ''accB''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
  • pGP1027: IPTG inducible expression, purification in ''E. coli'' without any tag, in [SW|pGP574], available in [SW|Jörg Stülke]'s lab
  • pGP2649: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844], available in [SW|Jörg Stülke]'s lab
  • pGP2650: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab
  • pGP2652: IPTG inducible expression, purification in ''E. coli'' with C-terminal Strep-tag, in [SW|pGP574], available in [SW|Jörg Stülke]'s lab
  • GFP fusion

  • GP1493 (mls, based on [SW|pGP1080]), available in [SW|Jörg Stülke]'s lab
  • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
  • FLAG-tag construct

  • GP1484 (''mls'', based on [SW|pGP1087]), available in [SW|Jörg Stülke]'s lab
  • References

    Reviews

  • 15952903,12121720,15968460
  • Original Publications

  • 7592499,8747466,24816803,28579978