SubtiBank SubtiBank
cggR [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

cggR [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]

repressor of the glycolytic gapA operon, DeoR family
locus
BSU33950
pI
5.68
mw
37.20 kDa
function
transcriptional regulator
product
central glycolytic genes regulator
essential
no
synonyms
yvbQ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.1|Glycolysis]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.5|Regulators of core metabolism]

    • [SW|Categories] containing this gene/protein

  • [SW|carbon core metabolism], [SW|transcription factors and their control], [SW|regulators of core metabolism]

    • This gene is a member of the following [SW|regulons]

  • [SW|CggR regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    3,482,752 -> 3,483,774

    The protein

    Catalyzed reaction/ biological activity

  • transcription repression of the glycolytic ''[protein|search|gapA]'' operon

    • Protein family

  • sorC transcriptional regulatory family (according to Swiss-Prot)

    • [SW|Domains]

  • DNA binding domain (H-T-H motif) (37–56)

    • Effectors of protein activity

  • fructose 1.6-bisphosphate [Pubmed|12622823] and dihydroxyacetone phosphate, glucose-6-phosphate and fructose-6-phosphate [Pubmed|18554327] act as inducer and result in release of CggR from the DNA

    • Structure

  • [PDB|2OKG] ( effector binding domain), [PDB|3BXH] (in complex with fructose-6-phosphate), complex with Fructose-6-Phosphate [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=65242 NCBI], effector binding domain [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=44226 NCBI]

    • [SW|Localization]

  • cytoplasma [Pubmed|20572937]

    • [SW|Interactions]

  • active as dimer (according to [Pubmed|20408793])

    • Expression and Regulation

    Operon

  • ''[protein|search|cggR]-[protein|search|gapA]-[protein|search|pgk]-[protein|search|tpi]-[protein|search|pgm]-[protein|search|eno]'' [Pubmed|11489127]
  • ''[protein|search|cggR]-[protein|search|gapA]'' [Pubmed|11489127]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|11489127]

    • Regulation

  • expression activated by glucose (77-fold) ([protein|search|CggR]) [Pubmed|12850135,12622823]

    • Regulatory mechanism

  • ''[protein|search|cggR]'': transcription repression [Pubmed|11489127]

    • Additional information

  • The primary mRNAs of the operon are highly unstable. The primary mRNA is subject to processing at the very end of the '[protein|search|cggR]' open reading frame. This results in stable mature '[protein|search|gapA]' and '[protein|search|gapA]-[protein|search|pgk]-[protein|search|tpiA]-[protein|search|pgm]-[protein|search|eno]' mRNAs. [PubMed|11489127] The processing event requires the [Rny|search|RNase Y] [PubMed|19193632].
  • The intracellular concentration of CggR is about 230 nM (according to [PubMed|20408793]).
  • The accumulation of the '[protein|search|cggR]-[protein|search|gapA]' mRNA is strongly dependent on the presence of the [protein|search|YkzW] peptide, due to stabilization of the mRNA [PubMed|20444087].
  • the mRNA is substantially stabilized upon depletion of [Rny|search|RNase Y] [PubMed|21815947]
  • exonucleolytic degradation of the '[protein|search|cggR]' RNA segment is performed by [protein|search|PnpA] [PubMed|25099370]

    • Biological materials

    Mutant

  • GP311 (in frame deletion), available in [SW|Jörg Stülke]'s lab
  • SM-NB7 (cggR-spc), available in [SW|Anne Galinier]'s and [SW|Boris Görke]'s labs

    • Expression vector

  • pGP705 (N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

    • lacZ fusion

  • pGP504 (in [protein|search|pAC7]), pGP509 (in [protein|search|pAC6]), available in [SW|Jörg Stülke]'s lab

    • Antibody

  • available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France

    • References

    Reviews

  • 20408793

    • Original Publications

  • 20462860,22190493,23280112,21815947,19193632,12850135,12622823,18186488,10799476,11489127,12123463,12634343,18052209,17293407,20361740,20444087,18554327,25099370