SubtiBank SubtiBank
secA

secA

preprotein translocase subunit (ATPase), required for membrane targeting of DivIVA, motor protein that drives preprotein translocation through the SecY-SecE-SecG channel
locus
BSU_35300
pI
5.34
mw
95.33 kDa
protein length
841 aa Sequence Blast
gene length
2526 bp Sequence Blast
function
protein secretion
product
preprotein translocase subunit (ATPase)
essential
yes
synonyms
div,div-341,ts-341

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.5|Protein secretion]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • Gene

    Coordinates
    3,628,310 3,630,835

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • terminal phenotype of CRISPRi knockdown mutant is moderate bending and a cessation of growth [Pubmed|27238023]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP ADP + Pi + preprotein translocation
  • required for membrane targeting of [protein|A8C0C2B7BCA6FFFF3811402D683FB7B99F7120A4|DivIVA] [Pubmed|24592260]
  • Protein family

  • SecA family (single member, according to UniProt)
  • [SW|Domains]

  • nucleotide binding domain, preprotein binding domain, IRA2 domain, scaffold domain, wing domain, IRA1 domain, C-terminal domain
  • [SW|Cofactors]

  • Mg
  • Effectors of protein activity

  • anionic phospholipids, preprotein, [protein|5350DC618130E013757EE667B9AFC5F2EC68EC08|SecY], signal peptides (even when added in trans) [Pubmed|19924216]
  • Structure

  • [PDB|1TF5] (open structure), [PDB|2IBM], [PDB|3DL8] (structure of the ([protein|5350DC618130E013757EE667B9AFC5F2EC68EC08|SecY]-[protein|1007EA15FB7AF81A44400287DB68BCC497158ACF|SecE]-[protein|D8851F453E5EF18D36F25834A8CB9C61EAF15E44|SecG])-[protein|AD7CD451B4AE638A719C91780339784A7FF0F248|SecA] complex [Pubmed|18923516]
  • [SW|Localization]

  • cell membrane (according to UniProt)
  • cytoplasm (according to UniProt)
  • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
  • Expression and Regulation

    Operons

    genes
    [gene|AD7CD451B4AE638A719C91780339784A7FF0F248|secA]-[gene|12E06FD3A36D95AE82E3C65788FA98178039592F|prfB]
    description
    [Pubmed|9882663]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9882663], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
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    Biological materials

    Mutant

  • BEC35300, a CRISPRi knockdown mutant, is available from the [http://bgsc.org/ Bacillus Genetic Stock Center]
  • References

    Reviews

  • 25212246,27890920,27799326
  • Original publications

  • 19933328,19924216,18923516,12873133,12242434,1385592,10816431,19850053,9880811,12218047,17981983,9882663,23484952,20574771,23167435,23794293,23852076,24592260,24786965,11021932,7851746,7894702,10074074,8497195,8440733,7706219,7642557,27336478,27238023,27795329,28041832,27802584,22354575