SubtiBank SubtiBank
mfd [2020-09-29 09:57:39]
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mfd [2020-09-29 09:57:39]

[SW|transcription]-repair coupling factor, eliminates genetic damage from transcriptionally active genes during sporulation, required for increased mutagenesis of lagging strand genes
133.59 kDa
protein length
1177 aa Sequence Blast
gene length
3534 bp Sequence Blast
promotes strand-specific DNA repair by displacing
[SW|transcription]-repair coupling factor

Genomic Context



  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW|Other proteins]
  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW|Transcription elongation/ termination]
  • Gene

    60,430 63,963

    Phenotypes of a mutant

  • in an ''mfd'' knock-out, the cell's ability to accumulate adaptive mutations in stationary phase is depressed [ PubMed]
  • strongly reduced stationary phase mutagenesis [Pubmed|27399782]
  • increased UV-induced mutagenesis via [protein|C87D33852F263A553CA747608E6200E4AD8344B5|PolY1]/ [protein|F0BC150F59730E64BA9AB3F979544EF6B5CC4E9E|PolY2]-mediated translesion synthesis [Pubmed|24118570]
  • the mutation suppresses the mucoid phenotype of ''[gene|86C729C7D5ABEE519ADC4A893940600BBB655EF1|motA]'' or ''[gene|FE56753D061344B0F10A6523F49C5C7356AA40B6|motB]'' mutants [Pubmed|24296669]
  • sensitive to blue light-induced DNA damage [pubmed|30054368]
  • sensitive to oxidative stress [pubmed|30691388]
  • suppression of lethality of [gene|C73E52D214E24F21696973B19B0A44CE785D6FBA|pcrA] inactivation [pubmed|32793628]
  • The protein

    Catalyzed reaction/ biological activity

  • promotes strand-specific DNA repair by displacing [SW|RNA polymerase] stalled at a nucleotide lesion and directing the (A)BC excinuclease to RNA damage site
  • is required for roadblock transcription repression by transcription factors with binding sites downstream of the promoter (as for [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA] [Pubmed|9535092] and [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY] [Pubmed|21699902])
  • required for the processing of genetic damage during [SW|sporulation] [Pubmed|24118570]
  • required for repair of DNA lesions during growth [pubmed|32041798]
  • promotes error-prone DNA repair during stress to promote genetic diversity [pubmed|32041798]
  • Protein family

  • N-terminal part: UvrB family (with [protein|DEFBDC2AB8E43FB06C65389160E8EACFE5FB9705|UvrB], according to UniProt)
  • C-terminal part: [SW|helicase family] (according to UniProt)
  • Paralogous protein(s)

  • [protein|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|RecU], [protein|24B1C2A663A5B7C45940272595868F9F05A04A7B|RecG]
  • [SW|Domains]

  • [SW|Helicase ATP-binding domain] (aa 638-799) (according to UniProt)
  • [SW|Helicase C-terminal domain] (aa 820-974) (according to UniProt)
  • Structure

  • [PDB|2EYQ] (Mfd from ''E. coli'') [Pubmed|16469698]
  • [PDB|3MLQ] (RNA polymerase interacting domain of ''Thermus thermophilus'' [protein|AD1F39823ABCE2A222259B879513752942C59590|Mfd] with the ''Thermus aquaticus'' [protein|EC32328548BFB59D9026ACE351C946DBBC0932BC|RpoB] beta1 domain) [Pubmed|20702425]
  • [SW|Localization]

  • cytoplasm (according to UniProt)
  • additional information

  • [protein|AD1F39823ABCE2A222259B879513752942C59590|Mfd] may interact with [SW|RNA polymerase] [pubmed|21710567]
  • Expression and Regulation


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    Biological materials


  • GP3519 (del kan), available in [SW|Jörg Stülke]'s lab
  • GP1167 (del ermC), available in [SW|Jörg Stülke]'s lab [Pubmed|22178973]
  • BKE00550 (''[gene|AD1F39823ABCE2A222259B879513752942C59590|mfd]''::''erm'', available in the BGSC, in [SW|Fabian Commichau]'s, and in [SW|Jörg Stülke]'s labs) [pubmed|28189581]
  • BKE00550 ([gene|AD1F39823ABCE2A222259B879513752942C59590|mfd]::erm trpC2) available at [ BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGGCCCCTCCTCTCTG, downstream forward: _UP4_TAAATTTTGTTACTCTCTGG
  • BKK00550 ([gene|AD1F39823ABCE2A222259B879513752942C59590|mfd]::kan trpC2) available at [ BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGGCCCCTCCTCTCTG, downstream forward: _UP4_TAAATTTTGTTACTCTCTGG
  • GFP fusion

  • GP1510 (spc, based on [SW|pGP1870], [SW|pGP1389]-derivative ), available in [SW|Jörg Stülke]'s lab
  • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab
  • Antibody

  • **
  • References


  • 17239578,15063847,17917874,8811173,7968917,15720542,16464004,19023283,22201950,22749141,22933559,26132790,29856930
  • Original publications

  • 8594198,20435731,104170,16950921,9535092,16469698,21699902,22178973,22248542,22960746,21710567,20702425,24296669,21145481,24118570,25326311,25961799,25713353,27399782,28700593,29536659,30054368,30691388,32041798,32793628