mhqO

hydroquinone-specific dioxygenase, confers resistence to methyl-hydroxyquinone

locus: BSU_05490

pI: 4.83

mw: 34.90 kDa

protein length: 312 aa

gene length: 939 bp

function: resistence to methyl-hydroxyquinone

product: hydroquinone-specific dioxygenase

essential: no

synonyms: ydfO

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

Gene

Coordinates: 597,114 598,052

The protein

Protein family

• [SW|extradiol ring-cleavage dioxygenase family] (according to UniProt)

Paralogous protein(s)

• [protein|7334017333600B876030056BE9247AD4E8996A1D|MhqA], [protein|A1CB3DCF2EC78B9B0585CA6AC6F5BB37B8AF0208|MhqE]

[SW|Domains]

• 2 [SW|VOC domain]s (aa 7-131, aa 152-269) (according to UniProt)

Structure

• [PDB|3OAJ]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|ACAFB2072E74CC059B492A31EF492028B96C569F|mhqO]-[gene|B4384C8C0874BAA7822E9B6DCAB57790018271D8|mhqP]
  description: [Pubmed|11532142]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|17725564], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|11532142], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulatory mechanism

  • [protein|997B828A99D6D8460712C18ED0CE566B285C22DC|MhqR]: repression, [Pubmed|17725564], in [regulon|997B828A99D6D8460712C18ED0CE566B285C22DC|MhqR regulon]

  regulation

  • induced by catechol and 2-methylhydroquinone ([protein|search|MhqR]) [Pubmed|17725564]

• genes: [gene|A0842DD5F1B13AC15C95F18751E7836DF31B262A|mhqN]-[gene|ACAFB2072E74CC059B492A31EF492028B96C569F|mhqO]-[gene|B4384C8C0874BAA7822E9B6DCAB57790018271D8|mhqP]
  description: [Pubmed|17725564]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|17725564], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|997B828A99D6D8460712C18ED0CE566B285C22DC|MhqR]: repression, [Pubmed|17725564], in [regulon|997B828A99D6D8460712C18ED0CE566B285C22DC|MhqR regulon]

  regulation

  • induced by catechol and 2-methylhydroquinone ([protein|search|MhqR]) [Pubmed|17725564]

Biological materials

Mutant

• MGNA-C154 (ydfO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2152 NBRP B. subtilis, Japan]
• BKE05490 ([gene|ACAFB2072E74CC059B492A31EF492028B96C569F|mhqO]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTAGCCATTTTTATCCGCT, downstream forward: _UP4_TGATTTGACACATAAAAAAT
• BKK05490 ([gene|ACAFB2072E74CC059B492A31EF492028B96C569F|mhqO]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTAGCCATTTTTATCCGCT, downstream forward: _UP4_TGATTTGACACATAAAAAAT

References

• 17407181,17725564,11532142,27965289