SubtiBank SubtiBank
guaA
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guaA

GMP synthetase (glutamine-hydrolysing)
locus
BSU_06360
pI
4.75
mw
57.78 kDa
protein length
513 aa Sequence Blast
gene length
1542 bp Sequence Blast
function
biosynthesis of GMP
product
GMP synthetase (glutamine-hydrolysing)
essential
no
ec
6.3.5.2
synonyms
guaB

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.1|Biosynthesis/ acquisition of purine nucleotides]
  • Gene

    Coordinates
    692,740 694,281

    The protein

    Catalyzed reaction/ biological activity

  • ATP + H2O + L-glutamine + XMP --> AMP + diphosphate + GMP + 2 H+ + L-glutamate (according to UniProt)
  • [SW|Domains]

  • [SW|Glutamine amidotransferase type-1 domain] (aa 8-198) (according to UniProt)
  • GMPS ATP-PPase domain (aa 199-388) (according to UniProt)
  • Effectors of protein activity

  • the enzyme is inhibited by guanosine tetraphosphate ([SW|stringent response]) [Pubmed|409404]
  • Structure

  • [PDB|2YWB] (from ''Thermus thermophilus hb8'', 45% identity, 57% similarity)
  • additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
  • Expression and Regulation

    Operons

    genes
    [gene|AC67305E4C165CC1D785E49B7CD8335346134376|guaA]
    description
    [Pubmed|1312531]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1312531], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
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    Biological materials

    Mutant

  • MGNA-A918 (yebB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/918 NBRP B. subtilis, Japan]
  • BKE06360 ([gene|AC67305E4C165CC1D785E49B7CD8335346134376|guaA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTTGTCACCTAATCTC, downstream forward: _UP4_TAAGAATCAATTAATGGAAA
  • BKK06360 ([gene|AC67305E4C165CC1D785E49B7CD8335346134376|guaA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTTGTCACCTAATCTC, downstream forward: _UP4_TAAGAATCAATTAATGGAAA
  • References

    Reviews

  • 11395405
  • Original publications

  • 1722815,1312531,409404,15378759,24682323,27120414,30974118