mrpB [2019-02-19 18:13:30]

Na⁺/H⁺ antiporter subunit, multiple resistance and pH homeostasis

locus: BSU31610

pI: 4.91

mw: 15.15 kDa

protein length: 143 aa

gene length: 432 bp

function: sodium export

product: Na⁺/H⁺ antiporter subunit

essential: yes

synonyms: yufU

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.2|Sodium uptake/ export]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,248,996 3,249,427

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Structure

• [PDB|6CFW] (from Pyrococcus furiosus, corresponds to aa 1 ... 104, 31% identity) [pubmed|29754813]

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|E968DECF869CAF7E247A518494AD70B554A7A25F|mrpA]-[gene|AAB15E7E3E3701AA3159370B85C151994244BA5B|mrpB]-[gene|D921139B18A665E9BB177C973FCF050A4BFFF97F|mrpC]-[gene|051D9BCC0CB05E39ED5825BB9BA77B8228EA055D|mrpD]-[gene|BF233467FAF95EEEE12A0D36C543DB357D8879EA|mrpE]-[gene|5CA2EA2D60FE4696903C16698C8329A20CBEBF72|mrpF]-[gene|B28796716C7E6C924F55176453DDBF9ADD0EF755|mrpG]
  description: [Pubmed|9274030,10198001]

Biological materials

Mutant

• MGNA-B558 (yufU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1557 NBRP B. subtilis, Japan]
• SM-GN9 (mrpB-spc), available in [SW|Anne Galinier]'s and [SW|Boris Grke]'s labs

References

Reviews

• 29218041

Original publications

• 11004162,9274030,10198001,19389778,17693497,18408029,29754813