function
utilization of sucrose, production of levan
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.19|Utilization of sucrose][category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins][SW|Categories] containing this gene/protein
[SW|utilization of specific carbon sources]This gene is a member of the following [SW|regulons]
[SW|DegU regulon], [SW|SacY regulon]Gene
Coordinates on the chromosome (coding sequence)
3,536,012 -> 3,537,433
The protein
Catalyzed reaction/ biological activity
Sucrose (6)-beta-D-fructofuranosyl-(2->)(n) alpha-D-glucopyranoside = glucose (6)-beta-D-fructofuranosyl-(2->)(n 1) alpha-D-glucopyranoside (according to Swiss-Prot)Protein family
glycosyl hydrolase 68 family (according to Swiss-Prot)Structure
[PDB|1PT2] (complex with sucrose), [PDB|1OYG][SW|Localization]
secreted (according to Swiss-Prot), extracellular (signal peptide) [Pubmed|18957862]Expression and Regulation
Operon
''[protein|search|sacB]-[protein|search|levB]-[protein|search|yveA]'' [Pubmed|11739774][SW|Sigma factor]
[protein|search|SigA] [Pubmed|2428811]Regulation
induction by sucrose (at high concentration) ([protein|search|SacY]) [Pubmed|3039303]Regulatory mechanism
[protein|search|SacY]: binding to a [SW|RNA switch] (transcriptional antitermination) [Pubmed|3039303][protein|search|DegU]-P: transcription activation [Pubmed|2428811]Biological materials
lacZ fusion
pGP564 (in [protein|search|pAC7]), a series of RAT mutations in [protein|search|pAC7], all available in [SW|Stülke] labReferences
Reviews
Original publications
6402497,2428811,18197985,18957862,2105292,11739774,3039303,22247149,26256357,26600431 
