SubtiBank SubtiBank
sacB [Mon Dec 14 2015 11:34:44 GMT+0100 (CET)]
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sacB [Mon Dec 14 2015 11:34:44 GMT+0100 (CET)]

levansucrase
locus
BSU34450
pI
6.00
mw
52.00 kDa
function
utilization of sucrose, production of levan
product
levansucrase
essential
no
ec
2.4.1.10
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.19|Utilization of sucrose]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]
  • [SW|Categories] containing this gene/protein

  • [SW|utilization of specific carbon sources]
  • This gene is a member of the following [SW|regulons]

  • [SW|DegU regulon], [SW|SacY regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    3,536,012 -> 3,537,433

    The protein

    Catalyzed reaction/ biological activity

  • Sucrose (6)-beta-D-fructofuranosyl-(2->)(n) alpha-D-glucopyranoside = glucose (6)-beta-D-fructofuranosyl-(2->)(n 1) alpha-D-glucopyranoside (according to Swiss-Prot)
  • Protein family

  • glycosyl hydrolase 68 family (according to Swiss-Prot)
  • Structure

  • [PDB|1PT2] (complex with sucrose), [PDB|1OYG]
  • [SW|Localization]

  • secreted (according to Swiss-Prot), extracellular (signal peptide) [Pubmed|18957862]
  • Expression and Regulation

    Operon

  • ''[protein|search|sacB]-[protein|search|levB]-[protein|search|yveA]'' [Pubmed|11739774]
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|2428811]
  • Regulation

  • induction by sucrose (at high concentration) ([protein|search|SacY]) [Pubmed|3039303]
  • Regulatory mechanism

  • [protein|search|SacY]: binding to a [SW|RNA switch] (transcriptional antitermination) [Pubmed|3039303]
  • [protein|search|DegU]-P: transcription activation [Pubmed|2428811]
  • Biological materials

    lacZ fusion

  • pGP564 (in [protein|search|pAC7]), a series of RAT mutations in [protein|search|pAC7], all available in [SW|Stülke] lab
  • References

    Reviews

  • 20735481
  • Original publications

  • 6402497,2428811,18197985,18957862,2105292,11739774,3039303,22247149,26256357,26600431