SubtiBank SubtiBank
alsS [Fri Nov 13 2015 17:21:21 GMT+0100 (CET)]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

alsS [Fri Nov 13 2015 17:21:21 GMT+0100 (CET)]

acetolactate synthase
locus
BSU36010
pI
5.00
mw
61.00 kDa
function
overflow metabolism
product
acetolactate synthase
essential
no
ec
2.2.1.6
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.5|Overflow metabolism]
  • [SW|Categories] containing this gene/protein

  • [SW|carbon core metabolism]
  • This gene is a member of the following [SW|regulons]

  • [SW|AlsR regulon], [SW|Rex regulon], [SW|stringent response]
  • Gene

    Coordinates on the chromosome (coding sequence)
    3,709,628 -> 3,711,340

    The protein

    Catalyzed reaction/ biological activity

  • 2 pyruvate = 2-acetolactate CO2 (according to Swiss-Prot)
  • Protein family

  • TPP enzyme family (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|search|IlvB], [protein|search|YdaP]
  • [SW|Cofactors]

  • Mg2 [Pubmed|25393087]
  • TPP [Pubmed|25393087]
  • Structure

  • [http://pdb.org/pdb/explore/explore.do?structureId=4rjj [4RJJ]] (complex wit TPP) [Pubmed|25393087]
  • Expression and Regulation

    Operon

  • ''[protein|search|alsS]-[protein|search|alsD]'' [Pubmed|7685336]
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|7685336]
  • Regulation

  • induction by acetate ([protein|search|AlsR]) [Pubmed|7685336]
  • repressed as long as terminal electron acceptors are available for respiration ([protein|search|Rex]) [Pubmed|16428414]
  • subject to positive stringent control upon lysine starvation [Pubmed|20081037]
  • Note: since acetate formation requires ''[protein|search|ackA]'' activation by [protein|search|CcpA] there is an indirect effect of [protein|search|CcpA] on the ''alsSD'' operon: the operon is not expressed in ''[protein|search|ccpA]'' mutants
  • Regulatory mechanism

  • stringent response: due to presence of adenines at 1 and 2 positions of the transcript [Pubmed|20081037]
  • [protein|search|AlsR]: transcription activation in the presence of acetate [Pubmed|7685336]
  • [protein|search|Rex]: transcription repression if the ratio NADH2/NAD is high [Pubmed|16428414]
  • Biological materials

    Mutant

  • 1A979 ( ''alsS''::''cat''), [Pubmed|7685336], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A979&Search=1A979 BGSC]
  • References

  • 19087206,10986270,20081037,16428414,19383131,7685336,16428414,19684168,24734205,22178965,25393087