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gyrB [2020-01-26 12:26:43]
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gyrB [2020-01-26 12:26:43]

DNA gyrase (subunit B)
locus
BSU_00060
pI
5.38
mw
71.32 kDa
protein length
638 aa Sequence Blast
gene length
1917 bp Sequence Blast
function
DNA supercoiling, control of [category|SW 3.1.1|replication] initiation
product
DNA gyrase (subunit B)
essential
yes
ec
5.99.1.3
synonyms
novA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.3|DNA condensation/ segregation]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

    • Gene

    Coordinates
    4,867 6,783

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • ATP-dependent breakage, passage and rejoining of double-stranded DNA (according to UniProt)
  • decreases [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA] association with the oriC and inhibits [category|SW 3.1.1|replication] initiation [pubmed|29396913]

    • Protein family

  • type II topoisomerase [protein|AA42910B71348EFEA4165CFFCEF1E497897B9DDC|GyrB] family (together with [protein|1901C52DCB24B3D53150F6926C14907AC1B75293|ParE]) (according to UniProt)

    • Paralogous protein(s)

  • [protein|1901C52DCB24B3D53150F6926C14907AC1B75293|ParE]

    • [SW|Domains]

  • [SW|TOPRIM domain] (aa 422-536) [Pubmed|9722641]

    • Modification

  • phosphorylated on Ser-400 [Pubmed|20509597]

    • Effectors of protein activity

  • novobiocin binds to the ATP binding site of GyrB and inhibits ATP hydrolysis and enzymatic activity, this results in [category|SW 3.1.1|replication] overinitiation [pubmed|29396913,6282812,368801]
  • an R138L mutation confers novobiocin resistance [pubmed|29396913]

    • Structure

  • [PDB|4I3H] (from ''Streptococcus pneumoniae'', 55% identity) [Pubmed|23965305]

    • [SW|Localization]

  • nucleoid-associated in actively growing cells [Pubmed|9539793]

    • additional information

  • GyrB is subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]

    • Expression and Regulation

    Operons

    genes
    [gene|FB1C21A94697E5B9D29C0F3B9C9F36E57BCBD237|yaaA]-[gene|EBE03AEC7EC594A3115A7A72194BDFF300AA0BFA|recF]-[gene|CBC76FB453569550F14FE5015C05D45985C04979|remB]-[gene|AA42910B71348EFEA4165CFFCEF1E497897B9DDC|gyrB]-[gene|B207C7328177924B16F1C606F667BEADF12CA069|gyrA]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2987848], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
    • view in new tab

    labs

  • [SW|Dagmar Klostermeier], University of Mnster, Germany [http://www.biozentrum.unibas.ch/klostermeier/index.html homepage]

    • References

    Reviews

  • 16936698

    • Original publications

  • 17981983,9539793,2987848,19666507,17320901,20509597,23475963,23965305,9722641,27557712,27793284,12767818,368801,6282812,29396913,8940171,7590259,10944214,6248518,6243420,225110,17334374,30517674