SubtiBank SubtiBank
gyrB
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

gyrB

DNA gyrase (subunit B)
locus
BSU00060
pI
5.38
mw
71.32 kDa
protein length
638 aa Sequence Blast
gene length
1914 bp Sequence Blast
function
DNA supercoiling, initation of replication cycle and DNA elongation
product
DNA gyrase (subunit B)
essential
yes
ec
5.99.1.3
synonyms
novA

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.3|DNA condensation/ segregation]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]
  • Gene

    Coordinates
    4,867 → 6,783

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP-dependent breakage, passage and rejoining of double-stranded DNA (according to Swiss-Prot)
  • Protein family

  • type II topoisomerase family (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|1901C52DCB24B3D53150F6926C14907AC1B75293|ParE]
  • [SW|Domains]

  • [SW|TOPRIM domain] (aa 422-536) [Pubmed|9722641]
  • Modification

  • phosphorylated on Ser-400 [Pubmed|20509597]
  • Structure

  • [PDB|4I3H] (from ''Streptococcus pneumoniae'', 55% identity) [Pubmed|23965305]
  • [SW|Localization]

  • nucleoid-associated in actively growing cells [Pubmed|9539793]
  • additional information

  • GyrB is subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
  • Expression and Regulation

    Operons

    genes
    [gene|FB1C21A94697E5B9D29C0F3B9C9F36E57BCBD237|yaaA]-[gene|EBE03AEC7EC594A3115A7A72194BDFF300AA0BFA|recF]-[gene|CBC76FB453569550F14FE5015C05D45985C04979|remB]-[gene|AA42910B71348EFEA4165CFFCEF1E497897B9DDC|gyrB]-[gene|B207C7328177924B16F1C606F667BEADF12CA069|gyrA]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2987848], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
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    Labs working on this gene/protein

  • [SW|Dagmar Klostermeier], University of Münster, Germany [http://www.biozentrum.unibas.ch/klostermeier/index.html homepage]
  • References

  • 17981983,9539793,2987848,19666507,17320901,20509597,23475963,23965305,9722641,27557712,27793284,12767818