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gltB [Sat May 28 2016 00:19:29 GMT+0200 (CEST)]
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gltB [Sat May 28 2016 00:19:29 GMT+0200 (CEST)]

small subunit of glutamate synthase
locus
BSU18440
pI
7.00
mw
54.00 kDa
function
glutamate biosynthesis
product
glutamate synthase (small subunit)
essential
no
ec
1.4.1.13
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.1|Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation]

    • [SW|Categories] containing this gene/protein

  • [SW|biosynthesis/ acquisition of amino acids], [SW|glutamate metabolism]

    • This gene is a member of the following [SW|regulons]

  • [SW|GltC regulon], [SW|FsrA regulon], [SW|TnrA regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,008,572 -> 2,010,053

    Phenotypes of a mutant

  • auxotrophic for glutamate

    • The protein

    Catalyzed reaction/ biological activity

  • 2 L-glutamate NADP = L-glutamine 2-oxoglutarate NADPH (according to Swiss-Prot) 2 L-glutamate NADP( ) <=> L-glutamine 2-oxoglutarate NADPH

    • Protein family

  • glutamate synthase family.

    • Paralogous protein(s)

  • none

    • [SW|Domains]

  • nucleotide binding domain (NADP) (299–313)

    • Structure

  • [PDB|2VDC] (the [protein|search|GltA]-[protein|search|GltB] complex of ''Azospirillum brasiliense'') [Pubmed|18199747]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • [SW|Interactions]

  • [protein|search|GltA]-[protein|search|GltB] [Pubmed|20933603]

    • Expression and Regulation

    Operon

  • ''[protein|search|gltA]-[protein|search|gltB]'' [Pubmed|12823818]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|2548995]

    • Regulation

  • see ''[protein|search|gltA]

    • Biological materials

    Mutant

  • GP807 (del ''[protein|search|gltA]-[protein|search|gltB]''::''tet'') , available in [SW|Jörg Stülke]'s lab
  • GP517 (ermC), available in [SW|Jörg Stülke]'s lab

    • Expression vector

  • pGP1119 (in [SW|pGP380], for SPINE, expression in ''B. subtilis''), available in [SW|Jörg Stülke]'s lab

    • lacZ fusion

  • see ''[protein|search|gltA]

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]
  • [SW|Jörg Stülke], University of Göttingen, Germany
  • [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
  • [SW|Fabian Commichau] University of Göttingen, Germany
  • [http://genmibio.uni-goettingen.de/index.php?id=130 Homepage]

    • References

    Reviews

  • 16143852,12859215,22625175,12859215

    • Original publications

  • 12823818,20933603,11029411,7559360,15150225,2548995,17183217,17608797,17134717,14523131,12823818,18326565,18199747,22389480,25755103