gltB [2017-02-27 15:02:04]

small subunit of glutamate synthase

locus: BSU18440

pI: 7.00

mw: 54.00 kDa

protein length: 493 aa

gene length: 1479 bp

function: glutamate biosynthesis

product: glutamate synthase (small subunit)

essential: no

ec: 1.4.1.13

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.1|Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation]

This gene is a member of the following [SW|regulons]

• [SW|GltC regulon], [SW|FsrA regulon], [SW|TnrA regulon]

Gene

Coordinates on the chromosome (coding sequence): 2,008,572 -> 2,010,053

Phenotypes of a mutant

• auxotrophic for glutamate

The protein

Catalyzed reaction/ biological activity

• 2 L-glutamate NADP = L-glutamine 2-oxoglutarate NADPH (according to Swiss-Prot) 2 L-glutamate NADP( ) <=> L-glutamine 2-oxoglutarate NADPH

Protein family

• glutamate synthase family.

Paralogous protein(s)

• none

[SW|Domains]

• nucleotide binding domain (NADP) (299–313)

Structure

• [PDB|2VDC] (the [protein|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|GltA]-[protein|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|GltB] complex of ''Azospirillum brasiliense'') [Pubmed|18199747]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation: [[this]]

Biological materials

Mutant

• GP807 (del ''[gene|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]-[gene|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|gltB]''::''tet'') , available in [SW|Jörg Stülke]'s lab
• GP517 (ermC), available in [SW|Jörg Stülke]'s lab

Expression vector

• pGP1119 (in [SW|pGP380], for SPINE, expression in ''B. subtilis''), available in [SW|Jörg Stülke]'s lab

lacZ fusion

• see ''[gene|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

Labs working on this gene/protein

• [SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]
• [SW|Jörg Stülke], University of Göttingen, Germany
• [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
• [SW|Fabian Commichau] University of Göttingen, Germany
• [http://genmibio.uni-goettingen.de/index.php?id=130 Homepage]

References

Reviews

• 16143852,12859215,22625175,12859215

Original publications

• 27223617,12823818,20933603,11029411,7559360,15150225,2548995,17183217,17608797,17134717,14523131,12823818,18326565,18199747,22389480,25755103