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divIVA [Tue May 03 2016 10:59:02 GMT+0200 (CEST)]
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divIVA [Tue May 03 2016 10:59:02 GMT+0200 (CEST)]

curvature sensitive membrane binding protein that recruits other proteins to the poles and the division septum, cell-division initiation protein (septum placement), part of the Min system (with Z ring placement)
locus
BSU15420
pI
4.00
mw
19.00 kDa
function
septum placement
product
cell-division initiation protein
essential
no
synonyms
ylmJ

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.1|The Min system]
  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
  • [SW|Categories] containing this gene/protein

  • [SW|cell division], [SW|membrane proteins], [SW|phosphoproteins], [SW|sporulation/ other]
  • This gene is a member of the following [SW|regulons]

  • [SW|Spo0A regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    1,612,521 -> 1,613,015

    Phenotypes of a mutant

  • Deletion of ''divIVA'' leads to filamentation and polar divisions that in turn cause a minicell phenotype. [Pubmed|9219999]
  • A ''divIVA'' mutant has a moderate [(Pubmed)|26735940] to severe [(Pubmed)|11445541] [SW|sporulation] defect.
  • The protein

    Catalyzed reaction/ biological activity

  • curvature sensitive membrane binding protein that recruits other proteins to the poles and the division septum
  • DivIVA is required for polar localisation of [protein|search|MinC]-[protein|search|MinD] via [protein|search|MinJ]. [Pubmed|19019154]
  • It also recruits [protein|search|RacA] to the distal pole of the prespore [Pubmed|12493822].
  • DivIVA may anchor [SW|SpoIIE] briefly to the assembling polar septum before [SW|SpoIIE] is subsequently released into the forespore membrane and recaptured at the polar septum [Pubmed|25101664]
  • required for the compartment-specific activation of [protein|search|SigF] [Pubmed|25101664]
  • activates [protein|search|PrkC] [Pubmed|25845974]
  • required for oriC placement during spore development [Pubmed|27059541]
  • Protein family

  • gpsB family (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|search|GpsB]
  • [SW|Domains]

  • the first 60 amino acids constitute a conserved lipid binding domain. [Pubmed|19478798]
  • the C-terminal domain is less conserved
  • multimerisation involves two coiled-coil motifs, one in the lipid binding domain, and the other one being present in the helical C-terminal domain [Pubmed|18388125] [Pubmed|23264578]
  • Modification

  • phosphorylated on Arg-102 [Pubmed|22517742]
  • The ''Mycobacterium'' DivIVA homologue Wag31 is phosphorylated at T73 [Pubmed|15985609]
  • DivIVA from ''Streptococcus pneumoniae'' is phosphorylated at Threonine 201 by the Ser/Thr protein kinase Sktp1. [Pubmed|20453092][Pubmed|22211696]
  • [SW|Cofactors]

  • not known
  • Effectors of protein activity

  • not known
  • Structure

  • [PDB|2WUJ] (N-terminal domain) [Pubmed|20502438]
  • [SW|Localization]

  • DivIVA forms a ring underneath the invaginating membrane at the site of cell division and is enriched at both cell poles [Pubmed|9219999].
  • forms rings at the division septum and patches at the cell poles [Pubmed|22108385]
  • membrane targeting requires [protein|search|SecA] [Pubmed|24592260]
  • assembles into a ring-like structure at the polar septum during [SW|sporulation] [Pubmed|25101664]
  • [SW|Interactions]

  • ([protein|search|MinC]-[protein|search|MinD])-[protein|search|MinJ]-[SW|DivIVA] [Pubmed|20352045], [protein|search|MinJ] binds to the N-terminal lipid-binding domain of [SW|DivIVA] [Pubmed|23264578]
  • [SW|DivIVA]-[protein|search|RacA], [protein|search|RacA] binds to the C-terminal domain of [SW|DivIVA] [Pubmed|23264578]
  • [SW|DivIVA]-[SW|DivIVA] [Pubmed|23264578]
  • [protein|search|Maf]-[SW|DivIVA] [Pubmed|21564336]
  • [SW|DivIVA]-[protein|search|ComN] [Pubmed|22582279]
  • [SW|DivIVA]-[protein|search|SecA] [Pubmed|24592260]
  • [SW|DivIVA]-[SW|SpoIIE] [Pubmed|25101664]
  • Expression and Regulation

    Operon

  • ''divIVA'' [Pubmed|16420366]
  • Regulation

  • repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]
  • constitutively expressed [Pubmed|23701187]
  • Regulatory mechanism

  • [SW|Spo0A]: transcription repression [Pubmed|14651647]
  • Biological materials

    Mutant

  • 4041 (''divIVA''::''tet''), available in [SW|Leendert Hamoen]'s, [SW|Jörg Stülke]'s, and [SW|Sven Halbedel] 's lab
  • GP1482 (chromosomal ''divIVA''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
  • Expression vector

  • DivIVA-Strep available [http://www.rki.de/DE/Content/Institut/OrgEinheiten/Abt1/FG11/AG_LM.html here]
  • pGP1497 (N-terminal Strep-tag fused to C-terminus of ''divIVA'', TEV-site, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
  • GFP fusion

  • divIVA-gfp fusions available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab
  • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Sven Halbedel]'s and [SW|Jörg Stülke]'s labs
  • Antibody

  • A polyclonal anti-DivIVA antiserum generated in rabbit is described here [Pubmed|11445541].
  • Labs working on this gene/protein

  • [SW|Leendert Hamoen], Centre for Bacterial Cell Biology, Newcastle upon Tyne, United Kingdom [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen x]
  • [SW|Imrich Barak], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]
  • [SW|Sven Halbedel], Robert Koch Institute [http://www.rki.de/DE/Content/Institut/OrgEinheiten/Abt1/FG11/AG_LM.html homepage]
  • References

    Reviews

  • 19654604,19884039,22722244,23182676
  • Original Publications

  • 22582279,19654604,19666580,9219999,19019154,15554965,12368265,11445541,10835369,12511520,14651647,19478798,19429628,11445541,9219999,9045828,20352045,20502438,11886553,21564336,22108385,22457634,22517742,22661688,23264578,23701187,23927765,24391905,24592260,24600441,25101664,25845974,27059541,26735940