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xlyB
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xlyB

N-acetylmuramoyl-L-alanine amidase
locus
BSU_12460
pI
9.80
mw
33.71 kDa
protein length
317 aa Sequence Blast
gene length
954 bp Sequence Blast
function
PBSX prophage-mediated lysis
product
N-acetylmuramoyl-L-alanine amidase
essential
no
ec
3.5.1.28
synonyms
yjpB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.1|Autolysis]
  • [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.1|PBSX prophage]
  • [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.6|Phage-related functions]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

    • Gene

    Coordinates
    1,317,535 1,318,488

    The protein

    Catalyzed reaction/ biological activity

  • Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)

    • Protein family

  • [SW|N-acetylmuramoyl-L-alanine amidase 2 family] (according to UniProt)

    • Paralogous protein(s)

  • [protein|C9C395A5F6A2C06EAC97F228C5C66D1628C937CF|CwlA], [protein|D5612882F1887BE342EF7072E71502382AB7289F|CwlH], [protein|50D7B97E4D3D41F74EF1942B85DE1DA4A33BEBB0|XlyA]

    • [SW|Domains]

  • contains a N-acetylglucosamine-polymer-binding [SW|LysM domain] [Pubmed|18430080]
  • contains an amidase_2 domain (like [protein|C1EB8D05386C1B35B8002239F5247CC6AB25F786|BlyA], [protein|C9C395A5F6A2C06EAC97F228C5C66D1628C937CF|CwlA], [protein|D5612882F1887BE342EF7072E71502382AB7289F|CwlH], [protein|A8AF827637B7208DFA101C9A2911BF9AB1AED08F|XlyB])
  • [SW|N-acetylmuramoyl-L-alanine amidase domain] (aa 40-142) (according to UniProt)
  • [SW|LysM domain] (aa 177-221) (according to UniProt)

    • Structure

  • [PDB|2L47] (from Bacillus phage Gamma, corresponds to aa 3 ... 166, 57% identity)

    • [SW|Localization]

  • secreted (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|A8AF827637B7208DFA101C9A2911BF9AB1AED08F|xlyB]
    description
    [pubmed|22383849]
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    additional information

  • the mRNA is very stable (> 15 min) [pubmed|12884008]

    • Biological materials

    Mutant

  • BKE12460 ([gene|A8AF827637B7208DFA101C9A2911BF9AB1AED08F|xlyB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE12460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCTATCATCTCTCCTTTT, downstream forward: _UP4_TAAATGAAAAGACGGAAGCT
  • BKK12460 ([gene|A8AF827637B7208DFA101C9A2911BF9AB1AED08F|xlyB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK12460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCTATCATCTCTCCTTTT, downstream forward: _UP4_TAAATGAAAAGACGGAAGCT

    • References

    Reviews

  • 18430080

    • Original publications

  • 12884008