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bcrC [Tue Dec 13 2016 11:16:37 GMT+0100 (CET)]
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bcrC [Tue Dec 13 2016 11:16:37 GMT+0100 (CET)]

undecaprenyl pyrophosphate phosphatase, produces the carrier lipid for cell wall synthesis, bacitracin resistance
locus
BSU36530
pI
9.00
mw
21.00 kDa
function
cell wall synthesis, resistance to bacitracin and oxidative stress
product
undecaprenyl pyrophosphate phosphatase
essential
no
ec
3.6.1.27
synonyms
ywoA

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.8|Biosynthesis of the carrier lipid undecaprenylphosphate]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.5|Biosynthesis of the carrier lipid undecaprenylphosphate]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.14|Resistance against toxins/ antibiotics/ based on similarity]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [SW|Categories] containing this gene/protein

  • [SW|cell wall synthesis], [SW|cell envelope stress proteins (controlled by SigM, V, W, X, Y)], [SW|heat shock proteins], [SW|resistance against toxins/ antibiotics], [SW|membrane proteins]
  • This gene is a member of the following [SW|regulons]

  • [SW|SigI regulon], [SW|SigM regulon], [SW|SigW regulon], [SW|SigX regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    3,758,547 -> 3,759,128

    Phenotypes of a mutant

  • increased susceptibility to rare earth elements [Pubmed|22904278]
  • a ''[SW|bcrC]'' ''[SW|uppP]'' double mutant is not viable (unless ''[SW|yodM]'' is artificially expressed) [Pubmed|27528508]
  • The protein

    Catalyzed reaction/ biological activity

  • undecaprenyl pyrophosphate --> undecaprenyl phosphate
  • Protein family

  • bcrC/ybjG/ywoA family (according to Swiss-Prot)
  • Effectors of protein activity

  • inhibited by heptaprenyl pyrophosphate which accumulates in a ''[protein|search|ytpB]'' mutant [Pubmed|24806199]
  • [SW|Localization]

  • cell membrane (according to Swiss-Prot)
  • Additional information

  • there is a second undecaprenyl pyrophosphate phosphatase in ''B. subtilis'', [protein|search|YubB]. However, [protein|search|YubB] seems to play only a minor role. [Pubmed|22904278]
  • Expression and Regulation

    Operon

  • ''bcrC
  • [SW|Sigma factor]

  • [protein|search|SigM] [Pubmed|17434969], [protein|search|SigI] [Pubmed|18156261], [protein|search|SigW] [Pubmed|11866510], [protein|search|SigX] [Pubmed|12399481]
  • Regulation

  • expression activated by glucose (3.3 fold) [Pubmed|12850135]
  • induced by heat shock ([protein|search|SigI]) [Pubmed|18156261]
  • induced by bacitracin
  • induced by oxidative stress
  • induced in the stationary phase due to the internal production of toxic peptides ([SW|SdpC], [SW|SkfA]) [Pubmed|26364265]
  • expression is reduced in a [protein|search|SigV] mutant [Pubmed|21926231]
  • Biological materials

    Mutant

  • MGNA-A214 (ywoA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/214 NBRP B. subtilis, Japan]
  • BKE36530 ''bcrC''::''erm trpC2'' is available from the Bacillus Genetic Stock Center
  • References

    Reviews

  • 27344142
  • Original publications

  • 17434969,18156261,22904278,14612242,12486040,12399481,17434969,15838020,14651641,24068241,24806199,21926231,15946938,26364265,27528508,27578312