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mreB [2017-05-09 15:36:36]
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mreB [2017-05-09 15:36:36]

cell shape-determining protein, forms filaments, the polymers control/restrict the mobility of the cell wall elongation enzyme complex, required for LytE activity
locus
BSU28030
pI
4.90
mw
35.79 kDa
protein length
337 aa Sequence Blast
gene length
1011 bp Sequence Blast
function
cell shape determination
product
cell shape-determining protein
essential
yes
synonyms
divIVB

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.2|Cell shape]
  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.7|Membrane dynamics]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • Gene

    Coordinates
    2,860,735 → 2,861,748

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • the mutation can be suppressed by inactivation of ''[gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA]'', ''[gene|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|ptsI]'', ''[gene|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]'' [Pubmed|19192185], by overexpression of [protein|7150BABA5086D5E2EB8102E4901A216F43576282|GlmR] [Pubmed|21320184], or by addition of 5 mM magnesium to the growth medium [Pubmed|15752190]
  • The protein

    Catalyzed reaction/ biological activity

  • forms straight filaments in a heterologous system [Pubmed|21091501]
  • polymerizes in the presence of millimolar divalent cations, binds and hydrolyzes GTP and ATP [Pubmed|19117023]
  • involved in the organization of ϕ29 DNA replication machinery in peripheral helix-like structures [Pubmed|22420858]
  • required for [protein|321F248C22D7283C0F3323F1F4069E36F8D7FE6C|LytE] activity [Pubmed|23869552]
  • Protein family

  • ftsA/mreB family (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|6ED386D49F973C1F6B1076974F54275DD583C9D3|Mbl], [protein|40829C3632E9DCEB9E1C14A674B4B217F504CFE0|MreBH]
  • Modification

  • acetylated at Lys-240, this is important for restricting cell wall growth and cell diameter [Pubmed|27376153]
  • Structure

  • [PDB|1JCF] (from ''Thermotoga maritima'', 57% identity) [Pubmed|11544518]
  • [SW|Localization]

  • during logarithmic growth, [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|MreB] forms discrete patches that move processively along peripheral tracks perpendicular to the cell axis [Pubmed|21636744]
  • forms transverse bands as cells enter the stationary phase [Pubmed|21636744]
  • forms antiparallel double filaments [Pubmed|24843005]
  • close to the inner surface of the cytoplasmic membrane [Pubmed|16950129]
  • reports on helical structures formed by MreB [Pubmed|16950129,20566861] seem to be misinterpretation of data [Pubmed|21636744]
  • normal localization depends on the presence of glucolipids, [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|MreB] forms irregular clusters in an ''[gene|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|ugtP]'' mutant [Pubmed|22362028]
  • during stationary phase: delocalized in the cytoplasm [Pubmed|26091431]
  • during development of [SW|genetic competence]: co-localizes with polar clusters of the late competence protein [protein|41DFEF8A6BC7E34A6681C26D92F2B08DC120A957|ComGA] ([protein|41DFEF8A6BC7E34A6681C26D92F2B08DC120A957|ComGA] sequesters [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|MreB]) [Pubmed|26091431]
  • Expression and Regulation

    Operons

    genes
    [gene|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB]-[gene|6A14D0C7FD94A838BAB3B74AA100EA678F1E4E34|mreC]-[gene|231C30963AFB863424C087CB722446D319976D65|mreD]-[gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]-[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]
    description
    [Pubmed|8459776]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, promoter p1, upstream of [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|Maf] [Pubmed|26091431], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, promoter p2, within [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|Maf] [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, promoter p4, upstream of [protein|8C94C9598A823A8405B3E1FA0124E21D90845B8E|MinC] [Pubmed|8459776], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]
  • regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: activation, [Pubmed|26091431], in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]
  • regulation

  • constitutively expressed [Pubmed|23701187]
  • view in new tab

    genes
    [gene|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf]-[gene|2BD11D71AA6D9EFAD8A209C8813CC8B059180C84|radC]-[gene|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB]-[gene|6A14D0C7FD94A838BAB3B74AA100EA678F1E4E34|mreC]-[gene|231C30963AFB863424C087CB722446D319976D65|mreD]-[gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]-[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, promoter p2 within [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|Maf] [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, promoter p1, upstream of [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|Maf] [Pubmed|26091431], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: activation, [Pubmed|11948146,11918817,21564336], in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]
  • regulation

  • constitutively expressed [Pubmed|23701187]
  • view in new tab

    Biological materials

    two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in the labs of [SW|Jeff Errington] and [SW|Boris Görke]
  • Antibody

  • available in the [SW|Jeff Errington] and [SW|Peter Graumann] labs
  • Labs working on this gene/protein

  • [SW|Jeff Errington], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington homepage]
  • [SW|Peter Graumann], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]
  • References

    Reviews

  • 17506674,17158703,15037301,21725336,20825347,21047262,22166997,22154164,22652894,21371139,25578957,25957405
  • Localization

  • 17064365,20566861,21636744,21636745,22069484,23783036,24010660,24843005,25402772,25676310
  • Other original publications

  • 15752190,11290328,12809607,1400224,7836311,16950129,19192185,19117023,19643765,19654094,19659933,8459776,11544518,20133608,21091501,21320184,18179421,21964069,22343529,22362028,22420858,21926231,23879732,23869552,24261876,24603761,26091431,17880425,20233306,27215790,27376153