transcription repressor of threonine biosynthetic gene
function
control of threonine biosynthesis
product
transcription repressor of threonine biosynthetic gene
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.6|Biosynthesis/ acquisition of lysine/ threonine][category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other][SW|Categories] containing this gene/protein
[SW|transcription factors and their control], [SW|biosynthesis/ acquisition of amino acids]Gene
Coordinates on the chromosome (coding sequence)
2,852,157 -> 2,852,600
The protein
Catalyzed reaction/ biological activity
control of the threonine biosynthetic genes (''[protein|search|hom]-[protein|search|thrC]-[protein|search|thrB]'' and ''[SW|thrD]'') [Pubmed|27260660]Protein family
UPF0735 family (according to Swiss-Prot)[SW|Domains]
putative DNA binding signature (aa 19-51)ACT (aspartate kinase-chorismate mutase-tyrA) domain (aa 70-145)Effectors of protein activity
lysine and cysteine (indirectly) inhibit DNA-binding activity of ThrR [Pubmed|27260660]Expression and Regulation
Operon
''[SW|spo0B]-[protein|search|obg]-[protein|search|thrR]-[protein|search|pheA]'' [Pubmed|22383849][SW|Sigma factor]
[protein|search|SigA] [Pubmed|2537815]Biological materials
Mutant
BKE27910 (''[protein|search|thrR]''::''erm'', available in the BGSC and in [SW|Jörg Stülke]'s and [SW|Fabian Commichau]'s labs) [Pubmed|27260660]Expression vector
pGP323 (N-terminal Strep-tag, in [SW|pGP172]) (available in [SW|Fabian Commichau]'s lab) [Pubmed|27260660]pBP620 (N-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP380]) (available in [SW|Fabian Commichau]'s lab) [Pubmed|27260660]pBP622 (C-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP382]) (available in [SW|Fabian Commichau]'s lab) [Pubmed|27260660]two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab [Pubmed|27260660]References
2537815,25777134,27260660 
