SubtiBank SubtiBank
yrrN
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yrrN

tRNA uridine hydroxylase (in complex with [protein|D4046AA4C4ACB6A0A2F2020CA1E659F65CAFE68D|YrrO])
locus
BSU_27350
pI
4.64
mw
34.92 kDa
protein length
309 aa Sequence Blast
gene length
930 bp Sequence Blast
function
introduction of 5-methoxyuridine modification in tRNA (U34)
product
tRNA uridine hydroxylase
essential
no
synonyms
trhP1
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]

    • Gene

    Coordinates
    2,794,147 2,795,076

    Phenotypes of a mutant

  • 50% reduction in mo5U tRNA levels [pubmed|31358606]

    • The protein

    Catalyzed reaction/ biological activity

  • hydroxylation of U34 in tRNA to give 5-hydroxyuridine (ho5U) (in complex with [protein|D4046AA4C4ACB6A0A2F2020CA1E659F65CAFE68D|YrrO]) with prephenate as hydroxyl donor, ho5U is the precursor for 5-methoxyuridine modification (by [protein|CE4F2965077F2A5882432D683520E09B70B9369A|TrmR]) [pubmed|31358606]

    • Protein family

  • peptidase U32 family (with [protein|D4046AA4C4ACB6A0A2F2020CA1E659F65CAFE68D|YrrO], according to UniProt)

    • Paralogous protein(s)

  • [protein|D4046AA4C4ACB6A0A2F2020CA1E659F65CAFE68D|YrrO]

    • Expression and Regulation

    Operons

    genes
    [gene|CE4F2965077F2A5882432D683520E09B70B9369A|trmR]-[gene|A3EC327DA14F188E88AF4221B51EE5950F882277|yrrN]-[gene|D4046AA4C4ACB6A0A2F2020CA1E659F65CAFE68D|yrrO]-[gene|A659D9894E777D8A99707CB750DF2918AEE06CE9|udk]-[gene|6FBF27C12B4B3DC2CE373E5199357042114471F8|greA]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-A008 (yrrN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/8 NBRP B. subtilis, Japan]
  • BKE27350 ([gene|A3EC327DA14F188E88AF4221B51EE5950F882277|yrrN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACGTTCACCTCTTCTT, downstream forward: _UP4_TATTAATCAAAAGGAGGTTA
  • BKK27350 ([gene|A3EC327DA14F188E88AF4221B51EE5950F882277|yrrN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACGTTCACCTCTTCTT, downstream forward: _UP4_TATTAATCAAAAGGAGGTTA

    • References

    Research papers

  • 31358606