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citG [Wed Aug 24 2016 13:19:20 GMT+0200 (CEST)]
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citG [Wed Aug 24 2016 13:19:20 GMT+0200 (CEST)]

fumarase
locus
BSU33040
pI
5.00
mw
50.00 kDa
function
TCA cycle
product
fumarate hydratase
essential
no
ec
4.2.1.2
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.4|TCA cycle]

    • [SW|Categories] containing this gene/protein

  • [SW|carbon core metabolism], [SW|most abundant proteins]

    • Gene

    Coordinates on the chromosome (coding sequence)
    3,389,024 -> 3,390,412

    The protein

    Catalyzed reaction/ biological activity

  • (S)-malate = fumarate H2O (according to Swiss-Prot)

    • Protein family

  • Fumarase subfamily (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|search|AnsB]

    • Structure

  • [PDB|3R6Q] (aspartase from ''Bacillus'' sp. YM55-1, 43% identity, 77% similarity) [Pubmed|21661762]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • [SW|Interactions]

  • [protein|search|Mdh]-[protein|search|CitG] [Pubmed|20933603]
  • [protein|search|CitG]-[protein|search|CitB] [Pubmed|20933603]

    • Expression and Regulation

    Operon

  • ''[protein|search|citG]'' [Pubmed|3923430]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|3130545,2509422,2509423], [protein|search|SigH] [Pubmed|3130545,2509422]

    • Regulation

  • constitutive

    • Additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

    • Biological materials

    Mutant

  • GP718 (spec), available in [SW|Jörg Stülke]'s lab
  • GP2340 (''[protein|search|citG]''::''kan''), Cre-recombinase is integrated in ''[protein|search|sacA]'',available in [SW|Jörg Stülke]'s lab
  • GP2341 (''[protein|search|citG]''::''lox72''), Cre-recombinase is integrated in ''[protein|search|sacA]'', available in [SW|Jörg Stülke]'s lab

    • Expression vector

  • pGP1122 (N-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP380]) (available in [SW|Jörg Stülke]'s lab)

    • lacZ fusion

  • pGP387 (in [protein|search|pAC7]), available in [SW|Jörg Stülke]'s lab

    • GFP fusion

  • GP1433 (spc, based on [SW|pGP1870]), available in the [SW|Stülke] lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • FLAG-tag construct

  • GP1132 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

    • Antibody

  • **

    • References

  • 2509422,3923430,3130545,2509423,21661762,20933603,15378759,6098632