pucM [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]

uricase

locus: BSU32460

pI: 5.00

mw: 13.00 kDa

function: purine utilization

product: uricase

essential: no

ec: 3.5.2.17

synonyms: yunM

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.1|Utilization of nucleotides]

[SW|Categories] containing this gene/protein

• [SW|utilization of nucleotides]

This gene is a member of the following [SW|regulons]

• [SW|PucR regulon], [SW|TnrA regulon]

Gene

Coordinates on the chromosome (coding sequence): 3,334,646 -> 3,334,990

The protein

Catalyzed reaction/ biological activity

• 5-hydroxyisourate H₂O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate (according to Swiss-Prot)

Protein family

• 5-hydroxyisourate hydrolase subfamily (according to Swiss-Prot)

Structure

• [PDB|2H0E] [Pubmed|16782815]

Expression and Regulation

Operon

• ''[protein|search|pucR]-[protein|search|pucJ]-[protein|search|pucK]-[protein|search|pucL]-[protein|search|pucM]'' [Pubmed|12823818]
• ''[protein|search|pucJ]-[protein|search|pucK]-[protein|search|pucL]-[protein|search|pucM]'' [Pubmed|12029039]

[SW|Sigma factor]

• ''[protein|search|pucJ]'': [protein|search|SigA] [Pubmed|12029039]

Regulation

• expressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|12823818]
• induced in the presence of purine nucleotides (inducer: allantoin) ([protein|search|PucR]) [Pubmed|12029039]

Regulatory mechanism

• [protein|search|TnrA]: transcription activation [Pubmed|12823818]
• [protein|search|PucR]: transcription activation [Pubmed|12029039]

References

• 11344136,12823818,12029039,16782815,20168977,25755103