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crh [Mon Aug 31 2015 13:56:58 GMT+0200 (CEST)]
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crh [Mon Aug 31 2015 13:56:58 GMT+0200 (CEST)]

"Carbon-flux regulating HPr", formerly known as "Catabolite repression HPr-like protein", control of flux through the harmful methylglyoxal pathway, minor cofactor of the CcpA transcription factor
locus
BSU34740
pI
4.70
mw
9.20 kDa
function
control of carbon flux
product
carbon-flux regulating HPr
essential
no
synonyms
yvcM
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]
    • syns
    yvcM

    [SW|Categories] containing this gene/protein

  • [SW|transcription factors and their control], [SW|phosphoproteins]

    • The protein

    Protein family

  • [PtsH|search|HPr] family

    • Paralogous protein(s)

  • [PtsH|search|HPr]

    • [SW|Domains]

  • [PtsH|search|HPr] domain (1–85)

    • Modification

  • phosphorylation on Ser46 by [protein|search|HprK] [Pubmed|9237995,21992469,22092971]

    • Structure

  • [PDB|2AK7] (dimeric Crh-Ser46-P)
  • [PDB|1ZVV] ([protein|search|CcpA]-Crh-DNA complex)
  • [PDB|2RLZ] (dimer)
  • [PDB|1MU4]
  • [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=24634 NCBI], dimer
  • [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=64943 NCBI], [protein|search|CcpA]-Crh-DNA complex
  • [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=52326 NCBI], dimeric phosphor-Crh
  • [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=39567 NCBI]

    • [SW|Interactions]

  • Crh-[protein|search|GapA] [Pubmed|17142398]
  • Crh-[protein|search|CcpA] [Pubmed|12972249]
  • [protein|search|HprK]-Crh [Pubmed|12009882]
  • [protein|search|Crh]-[protein|search|MgsA] (only non-phosphorylated [protein|search|Crh] interacts with [protein|search|MgsA]) [Pubmed|21992469]

    • Additional information

  • Crh does not possess the phosphorylation site used for [SW|PTS] phosphotransfer (His-15 in [protein|search|PtsH]), it can only be phosphorylated on Ser-46

    • Expression and Regulation

    Operon

  • ''[protein|search|yvcI]-[protein|search|yvcJ]-[protein|search|yvcK]-[protein|search|yvcL]-[protein|search|crh]-[protein|search|yvcN]'' [Pubmed|9237995]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|16272399]

    • Regulation

  • very weak stimuation of expression by citrate and succinate [Pubmed|16272399]

    • Biological materials

    Mutant

  • GP860 (aphA3) [Pubmed|21992469], QB7097 (spc), available in [SW|Jörg Stülke]'s lab

    • Expression vector

  • pGP641 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab
  • pGP734 (C-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP382]), available in [SW|Jörg Stülke]'s lab

    • lacZ fusion

  • see ''[protein|search|yvcI]

    • two-hybrid system

  • ''[protein|search|crh]'', ''crh(Ser46Asp)'', ''crh(Ser46Ala)'' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Boris Görke], University of Göttingen, Germany
  • [http://wwwuser.gwdg.de/~genmibio/goerke.html Homepage]
  • [SW|Anne Galinier], University of Marseille, France
  • [SW|Wolfgang Hillen], Erlangen University, Germany [http://www.biologie.uni-erlangen.de/mibi/index2.html Homepage]
  • [SW|Richard Brennan], Houston, Texas, USA [http://www.mdanderson.org/departments/biochem/display.cfm?id=556ef368-6c81-4043-b74f350d41dd06cb&method=displayfull&pn=a8427ebd-d0ff-11d4-80fd00508b603a14 Homepage]

    • References

  • 12972249,9973552,9237995,16272399,15126459,10217795,17142398,16316990,12009882,10589728,12670692,11916384,11361076,11361074,18320329,18284240,16411239,21992469,22092971