SubtiBank SubtiBank
spoIVA [Thu Sep 24 2015 19:23:01 GMT+0200 (CEST)]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

spoIVA [Thu Sep 24 2015 19:23:01 GMT+0200 (CEST)]

ATPase, spore coat morphogenetic protein, anchors the spore coat to the spore surface via SpoVM
locus
BSU22800
pI
4.00
mw
55.00 kDa
function
spore cortex formation and coat assembly
product
ATPase, basement layer protein for spore coat assembly
essential
no
synonyms
spoVP

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.1|Spore coat proteins] → [category|SW 4.2.1.1.1|Class I]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
  • [SW|Categories] containing this gene/protein

  • [SW|sporulation proteins]
  • This gene is a member of the following [SW|regulons]

  • [SW|SigE regulon], [SW|SpoIIID regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    2,386,195 -> 2,387,673

    Phenotypes of a mutant

  • the spore coat does not localize to the spore surface but self-assembles into aggregates in the mother cell cytoplasm [Pubmed|22171814]
  • The protein

    Catalyzed reaction/ biological activity

  • uses ATP hydrolysis to drive self-assembly into static filaments [Pubmed|23267091]
  • ATP hydrolysis drives polymerization of a nucleotide-free filament [Pubmed|23267091]
  • ploymerization depends on a critical threshold concentration of SpoIVA that is only achieved once the protein is recruited to the surface of the developing spore [Pubmed|23267091]
  • [SW|Domains]

  • contains a Walker A ATPase domain
  • [SW|Localization]

  • innermost protein of the spore coat basement [Pubmed|22171814]
  • [SW|Interactions]

  • [SW|SpoIVA]-[SW|SpoVID] [Pubmed|19702880,22262582]
  • [SW|SpoIVA]-[protein|search|SafA] [Pubmed|19702880,22262582]
  • [SW|SpoIVA]-[SW|SpoVM]-[SW|SpoVID] [Pubmed|19775244]
  • [SW|CmpA]-[SW|SpoIVA] (for [SW|ClpX]-[SW|ClpP]-dependent degradation of [SW|SpoIVA]) [Pubmed|26387458]
  • [SW|ClpX]-[SW|SpoIVA] (for [SW|ClpX]-[SW|ClpP]-dependent degradation of [SW|SpoIVA]) [Pubmed|26387458]
  • Expression and Regulation

    Operon

  • ''spoIVA'' [Pubmed|1729246]
  • [SW|Sigma factor]

  • [protein|search|SigE] [Pubmed|1729246,15699190]
  • Regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE], [SW|SpoIIID]) [Pubmed|1729246,15699190,15383836]
  • Regulatory mechanism

  • [SW|SpoIIID]: transcription repression [Pubmed|15383836]
  • References

    Reviews

  • 22192522,23202530
  • Original publications

  • 8748030,15699190,1729246,8936302,1729247,9922240,7592342,8299942,1691789,12644503,18691972,17427285,19775244,22171814,15383836,23267091,19702880,22262582,24810258,25854653,26387458