yhcS

sortase

locus: BSU_09200

pI: 7.17

mw: 21.83 kDa

protein length: 198 aa

gene length: 597 bp

function: anchoring of proteins to the cell wall

product: sortase

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.5|Protein secretion]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 994,998 995,594

The protein

Catalyzed reaction/ biological activity

• C-terminal anchoring of proteins to the cell wall

Protein family

• bacterial sortase family (with [protein|B17670494CC6F7C1138F4B4ECC0FEE8FC15609A2|YwpE], according to UniProt)

Structure

• [PDB|2LN7] (B. anthracis SrtD, corresponds to aa 69 - 195 of YhcS, 44% identity) [pubmed|22974341]

[SW|Localization]

• membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|DAFBC470DA0FA437B117716DE899A8D3EC454672|yhcR]-[gene|A24243129860F1B043EBE1358FAEA57BED111BBC|yhcS]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-A661 (yhcS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/661 NBRP B. subtilis, Japan]
• BKE09200 ([gene|A24243129860F1B043EBE1358FAEA57BED111BBC|yhcS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATGAATAGTGGAATAACTT, downstream forward: _UP4_TAAAAAGCCGAGGCAGTGCC
• BKK09200 ([gene|A24243129860F1B043EBE1358FAEA57BED111BBC|yhcS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATGAATAGTGGAATAACTT, downstream forward: _UP4_TAAAAAGCCGAGGCAGTGCC

References

Reviews

• 22026821,25845800

Original publications

• 21800427,21906378,22020651,22974341