SubtiBank SubtiBank
ywcH
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ywcH

similar to monooxygenase
locus
BSU_38100
pI
5.94
mw
36.40 kDa
protein length
333 aa Sequence Blast
gene length
1002 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
ipa-44d

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]
  • Gene

    Coordinates
    3,910,302 3,911,303

    The protein

    Paralogous protein(s)

  • [protein|A36E7D50E2EBD0F0109491FF2CAD2CA84372B2CF|YvbT], [protein|EDC3565D90D7059A4A4C66A13EB568420F6CECD4|CmoO], [protein|1432F56AD3D2B2BD82D8759CE4DC42C0D40C49C0|YceB]
  • Structure

  • [PDB|4US5] (from Streptomyces bottropensis, 39% identity) [pubmed|24554499]
  • Expression and Regulation

    Operons

    genes
    [gene|5B95F7CFEFCB954FCBC11087F030A1D777F34B7A|nfrA]-[gene|A1DCFF8D225C87197104742E72A0D4F238BC30EB|ywcH]
    description
    [Pubmed|10913069]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10913069], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|9535080], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]
  • regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [Pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [Pubmed|29271514,12642660,19575568], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]
  • regulation

  • repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]
  • induced by cell wall stress ([protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]) [pubmed|29271514]
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    Biological materials

    Mutant

  • MGNA-B233 (ywcH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1232 NBRP B. subtilis, Japan]
  • BKE38100 ([gene|A1DCFF8D225C87197104742E72A0D4F238BC30EB|ywcH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCTACCACCTCATCCT, downstream forward: _UP4_TGATCTTTAAAAAGCCCTGC
  • BKK38100 ([gene|A1DCFF8D225C87197104742E72A0D4F238BC30EB|ywcH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCTACCACCTCATCCT, downstream forward: _UP4_TGATCTTTAAAAAGCCCTGC
  • References

  • 10913069,9535080,14651647,24554499