yabA

inhibitor of DnaA oligomerization, tethers DnaA to the polymerase-clamp protein DnaN

Locus: BSU_00330

Isoelectric point: 6.07

Molecular weight: 13.96 kDa

Protein length: 119 aa

Gene length: 360 bp

Function: positioning of DnaA

Product: negative regulator of replication initiation

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA replication
• Groups of genes → Phosphoproteins → Phosphorylation on a Thr residue

Gene

Coordinates: 42,499 42,858

Phenotypes of a mutant

• strongly reduced growth rate in minimal medium PubMed
• over-initiation of replication PubMed
• a whiA yabA double mutant is not viable PubMed

The protein

Catalyzed reaction/ biological activity

• negative control of replication initiation PubMed
• associates with the oriC region in a DnaA-dependent manner, this limits the amount of DnaA that can bind the oriC region PubMed
• YabA inhibits co-operative DNA binding by DnaA PubMed
• inhibits oligomerization and helix formation of DnaA PubMed

Protein family

• YabA family (single member, according to UniProt)

Domains

• leucine zipper

Modification

• phosphorylated on Thr-71 by YabT PubMed

Cofactors

• Zinc PubMed

Effectors of protein activity

• DnaN overexpression or release from the replisome decreases association of YabA with oriC, increases association of DnaA with oriC PubMed

Structure

• 5DOL PubMed

Localization

• forms foci in the midcell region, this depends on the interaction with DnaA and DnaN PubMed
• co-localizes with the origin region and replication forks throughout the cell cycle PubMed

Expression and Regulation

Operons

• Genes: xpaC-yaaN-yaaO-tmk-darA-yaaR-holB-yaaT-yabA-trmN6-yazA-yabC
  Description: PubMed

• Genes: darA-yaaR-holB-yaaT-yabA-trmN6-yazA-yabC
  Description: PubMed

  Regulation

  • expressed during growth and the transition phase, expression is erduced in stationary phase PubMed

• Genes: tmk-darA-yaaR-holB-yaaT-yabA-trmN6-yazA-yabC
  Description: PubMed

Biological materials

Mutant

• MGNA-B900 (yabA::erm), available at the NBRP B. subtilis, Japan
• BKE00330 (yabA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTATCCAAGGTTCCACACC, downstream forward: _UP4_TAGGGAAAAGGCTTCCGCAT
• BKK00330 (yabA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTATCCAAGGTTCCACACC, downstream forward: _UP4_TAGGGAAAAGGCTTCCGCAT

Labs working on this gene/protein

• Philippe Noirot, Jouy-en-Josas, France Homepage
• Alan Grossman, Cambridge, MA, USA Homepage

References

Reviews

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Original publications

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