spoIIR

required for processing and compartmentalization of pro-[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]

locus: BSU_36970

pI: 4.74

mw: 25.20 kDa

protein length: 224 aa

gene length: 675 bp

function: control of [protein|search|SigE ]activation

product: morphological chekcpoint for [protein|search|SigE ]processing

essential: no

synonyms: ipc-27d,csfX

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.2|Control of sigma factors]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

Gene

Coordinates: 3,794,676 3,795,350

The protein

Catalyzed reaction/ biological activity

• interacts with and activates the pro-[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE] protease [protein|221829E084DA78ACE1C2DFCEB6E59EDA534FF55D|SpoIIGA] in the intermembrane space between the forespore and the mother cell [Pubmed|22111992]

Modification

• likely acylated on Thr-27 [Pubmed|22111992]

[SW|Localization]

• localizes initially to the forespore septal membrane (in a [protein|221829E084DA78ACE1C2DFCEB6E59EDA534FF55D|SpoIIGA]-dependent manner), is then exported to the intermembrane space between the forespore and the mother cell [Pubmed|22111992]

Expression and Regulation

Operons

• genes: [gene|9F338225E1286D530EAAAB504DEC8D5AFA06AC8D|spoIIR]
  description: (according to [http://dbtbs.hgc.jp/COG/prom/spoIIR.html DBTBS]) null

  sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|16497325], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

  regulation

  • expressed early during sporulation in the forespore ([protein|search|SigF]) [Pubmed|16497325,15699190]

Biological materials

Mutant

• BKE36970 ([gene|9F338225E1286D530EAAAB504DEC8D5AFA06AC8D|spoIIR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGGTCCCCACCGTTCCT, downstream forward: _UP4_TAATCATCTGGTCTAAAAAT
• BKK36970 ([gene|9F338225E1286D530EAAAB504DEC8D5AFA06AC8D|spoIIR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK36970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGGTCCCCACCGTTCCT, downstream forward: _UP4_TAATCATCTGGTCTAAAAAT

References

Reviews

• 31350897

Original Publications

• 16497325,7585939,15044948,11849534,19578359