sivC

inhibitor of entry into sporulation via [protein|2111AC1AE49D1006E10DC127BF5B7A0327DE94A7|KinB] or [protein|A656321846B2E0D1F39B528E2D8B8E620CCD1148|KinC]

locus: BSU_15960

pI: 8.61

mw: 17.60 kDa

protein length: 161 aa

gene length: 486 bp

function: control of entry into sporulation via the phosphorelay

product: inhibitor of entry into sporulation

essential: no

synonyms: ylqB

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay] → [category|SW 3.4.7.2|Proteins controlling the activity of the kinases]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay] → [category|SW 4.2.2.2|Proteins controlling the activity of the kinases]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 1,671,166 1,671,651

The protein

Catalyzed reaction/ biological activity

• inhibits [protein|2111AC1AE49D1006E10DC127BF5B7A0327DE94A7|KinB] or [protein|A656321846B2E0D1F39B528E2D8B8E620CCD1148|KinC] activity [Pubmed|23335417]

[SW|Localization]

• extracellular (signal peptide) [Pubmed|18957862]

Expression and Regulation

Operons

• genes: [gene|9D320646C56FA83962BFBE73E22F2BCF2F0788B1|sivC]
  description: [Pubmed|15033535]

  sigma factors

  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|15033535], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|18840696], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • repressed by casamino acids [Pubmed|12107147]

Biological materials

Mutant

• MGNA-B140 (ylqB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1139 NBRP B. subtilis, Japan]
• BKE15960 (''[gene|9D320646C56FA83962BFBE73E22F2BCF2F0788B1|sivC]''::''erm'', available in the BGSC and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]
• GP1823(''[gene|9D320646C56FA83962BFBE73E22F2BCF2F0788B1|sivC]''::''erm'', available in [SW|Jörg Stülke]'s lab)
• BKE15960 ([gene|9D320646C56FA83962BFBE73E22F2BCF2F0788B1|sivC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAATCCTCCTTAAAA, downstream forward: _UP4_TAAAAAAGAAAAAGGCCCCA
• BKK15960 ([gene|9D320646C56FA83962BFBE73E22F2BCF2F0788B1|sivC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAATCCTCCTTAAAA, downstream forward: _UP4_TAAAAAAGAAAAAGGCCCCA

References

• 18957862,12107147,15033535,18840696,23335417