coaX [2019-07-19 10:15:11]

type III pantothenate kinase

locus: BSU_00700

pI: 8.37

mw: 26.07 kDa

protein length: 258 aa

gene length: 777 bp

function: biosynthesis of coenzyme A

product: pantothenate kinase

essential: no

ec: 2.7.1.33

synonyms: yacB

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.4|Biosynthesis of coenzyme A]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]

Gene

Coordinates: 79,092 79,868

The protein

Catalyzed reaction/ biological activity

• ATP + (R)-pantothenate = ADP + (R)-4'-phosphopantothenate (according to Swiss-Prot)

Protein family

• type III pantothenate kinase family (single member, according to UniProt)

Structure

• [PDB|2H3G] (from ''Bacillus anthracis str. ames'', 75% identity, 88% similarity) [Pubmed|17323930]

[SW|Localization]

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|9B8EAD4EDDA97526D4462F6F3205F81FECD039DE|coaX]-[gene|D579A7E4D8C3DEE4F85586C5F721CAD5FE2BCA75|yacC]-[gene|548926987569033AA0C40610B8E80FB2BB8E1E3A|yacD]-[gene|D1FC976597E5583E40A4ED7234FBCA743AB01354|cysK]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]

  regulatory mechanism

  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [pubmed|30480837], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]

  regulation

  • induced by heat stress ([protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]) [pubmed|30480837]

• genes: [gene|9B8EAD4EDDA97526D4462F6F3205F81FECD039DE|coaX]-[gene|D579A7E4D8C3DEE4F85586C5F721CAD5FE2BCA75|yacC]
  description: [Pubmed|18179421]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]

Biological materials

Mutant

• MGNA-B923 (yacB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1922 NBRP B. subtilis, Japan]
• BKE00700 ([gene|9B8EAD4EDDA97526D4462F6F3205F81FECD039DE|coaX]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACCTCTATCACCACTTTT, downstream forward: _UP4_GGAAGTGTATAGGAGGTTTA
• BKK00700 ([gene|9B8EAD4EDDA97526D4462F6F3205F81FECD039DE|coaX]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACCTCTATCACCACTTTT, downstream forward: _UP4_GGAAGTGTATAGGAGGTTTA

References

• 15795230,18179421,24784177