c-di-AMP specific phosphodiesterase
function
control of sporulation initiation, cell wall homeostasis
product
c-di-AMP phosphodiesterase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.3|Metabolism of signalling nucleotides][category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.3|Targets of (p)ppGpp][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins][SW|Categories] containing this gene/protein
[SW|metabolism of signalling nucleotides], [SW|membrane proteins]Gene
Coordinates on the chromosome (coding sequence)
4,163,643 -> 4,165,622
Phenotypes of a mutant
inactivation of ''[protein|search|gdpP]'' strongly restores beta-lactam resistance in a ''[protein|search|sigM]'' mutant [Pubmed|22211522]a ''[SW|gdpP] [SW|pgpH]'' double mutant acquires suppressor mutations in ''[SW|cdaA]'' [Pubmed|26240071]a ''[SW|gdpP] [SW|pgpH]'' double mutant is defective in [SW|biofilm formation] [Pubmed|27252699]The protein
Catalyzed reaction/ biological activity
cyclic dinucleotide phosphodiesterase, hydrolysis of di-cyclic AMP [Pubmed|19901023][SW|Domains]
two transmembrane domains at the N-terminus [Pubmed|19901023]a domain that shares minimum sequence homology with Heme-binding [PAS domain|Per-ARNT-Sim (PAS) domains] (80 aa) [Pubmed|21257773]degenerate [SW|GGDEF domain] (aa 173 - 301) [Pubmed|19901023]DHH/ DHHA1 phosphodiesterase domain [Pubmed|19901023]Effectors of protein activity
ppGpp inhibits cyclic dinucleotide phosphodiesterase activity [Pubmed|19901023]Heme binding suppresses phosphodiesterase activity [Pubmed|21257773]Structure
[PDB|2M1C] (N-terminal PAS domain of GdpP from ''Geobacillus thermodenitrificans'') [Pubmed|23504327]Expression and Regulation
Operon
''[protein|search|yybS]-[protein|search|gdpP]-[protein|search|rplI]'' (according to [http://dbtbs.hgc.jp/COG/prom/yybST-rplI.html DBTBS])Additional information
there is an internal [protein|search|SigD]-dependent antisense transcript (as-'[protein|search|gdpP]') [PubMed|22956758]Biological materials
Mutant
GP998 (''[protein|search|gdpP]''::''spc''), available in [SW|Jörg Stülke]'s lab [Pubmed|23192352]GP2040 (''[protein|search|gdpP]''::''spc'' ''[protein|search|pgpH]''::''erm'' without terminator, available in [SW|Jörg Stülke]'s lab) [Pubmed|26240071]References
Reviews
Original publications
19901023,21257773,21566650,23504327,22211522,22956758,25616256,26240071,27252699 GdpP homologs in other organisms
