bmrD

multidrug [SW|ABC transporter ](ATP-binding protein), also involved in the signalling pathway to activate [protein|search|KinA ]at the onset of [SW|sporulation]

locus: BSU_09720

pI: 7.08

mw: 76.12 kDa

protein length: 673 aa

gene length: 2022 bp

function: multiple antibiotic resistance

product: multidrug [SW|ABC transporter ](ATP-binding protein)

essential: no

synonyms: yheH

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.2|Exporters] → [category|SW 1.2.1.2.1|Efflux of antibiotics]
• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.3|Regulatory ABC transporters]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,047,072 1,049,093

The protein

Protein family

• [SW|ABC transporter superfamily] (according to UniProt)

Paralogous protein(s)

• [protein|0EB4D3E1B9AF39ECCBD79EB559FAE5BB0EE4C156|YgaD]

[SW|Domains]

• has both a membrane-spanning and an ATP-binding domain [Pubmed|10092453]
• [SW|ABC transmembrane type-1 domain] (aa 18-398) (according to UniProt)
• [SW|ABC transporter domain] (aa 430-664) (according to UniProt)

Structure

• [PDB|5MKK] (TmrAB complex from Thermus thermophilus, 38% identity) [pubmed|28069938]

[SW|Localization]

• cell membrane [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|E9956A958C66C4114EB974EA6F9BF30CD951034A|bmrB]-[gene|67C0C9EA17EF953554D3D57CD0D285246F50C1AD|bmrC]-[gene|9B2766D2F8892AA17DB480216570C81C33355C3B|bmrD]
  description: [Pubmed|25217586]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675,25217586], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|E9956A958C66C4114EB974EA6F9BF30CD951034A|BmrB]: attenuation, [Pubmed|25217586], in [regulon|E9956A958C66C4114EB974EA6F9BF30CD951034A|BmrB regulon]

  regulation

  • ''[protein|search|bmrB]-[protein|search|bmrC]-[protein|search|bmrD]'' expression only occurs during the late-exponential and stationary growth stages ([protein|search|AbrB]) [Pubmed|25217586]

Biological materials

Mutant

• MGNA-B491 (yheH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1490 NBRP B. subtilis, Japan]
• BKE09720 ([gene|9B2766D2F8892AA17DB480216570C81C33355C3B|bmrD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCTCCATAACGTTTTTCCTA, downstream forward: _UP4_TAACGCTCAAAAACCCAAAA
• BKK09720 ([gene|9B2766D2F8892AA17DB480216570C81C33355C3B|bmrD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCTCCATAACGTTTTTCCTA, downstream forward: _UP4_TAACGCTCAAAAACCCAAAA

References

• 19167342,10092453,16487324,25217586,21602923,29507890,28069938