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thiV [2018-05-04 14:33:12]
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thiV [2018-05-04 14:33:12]

thiamine ABC transporter (membrane protein)
locus
BSU13230
pI
9.89
mw
21.02 kDa
protein length
199 aa Sequence Blast
gene length
597 bp Sequence Blast
function
thiamine uptake
product
thiamine ABC transporter (membrane protein)
essential
no
synonyms
ykoE

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.3|ECF transporter] → [category|SW 1.2.3.3|Class I ECF transporter]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.3|Biosynthesis/ acquisition of thiamine]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • Gene

    Coordinates
    1,390,439 → 1,391,038

    The protein

    [SW|Localization]

  • membrane associated [Pubmed|18763711]
  • Expression and Regulation

    Operons

    genes
    [gene|57A953B5D71F3FD8269EAF2366B8E2C7A8C79483|thiU]-[gene|9AF27E4CE34B5C4036840A27D93B13213E3324DD|thiV]-[gene|EF69891D9CF0CE57367B38FB3C69DDE1B689FCB7|thiW]-[gene|236095EDE02EC9EA7768D9D30CED2345D3B7A1A9|thiX]
    description
    [Pubmed|16291685]

    regulatory mechanism

  • [protein|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box]: [SW|RNA switch], via [SW|RNA switch], in [regulon|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box regulon]
  • regulation

  • repressed by thiamine ([protein|search|Thi-box]) [Pubmed|12376536]
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    Biological materials

    Mutant

  • MGNA-A756 (ykoE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/756 NBRP B. subtilis, Japan]
  • BKE13230 (Δ[gene|9AF27E4CE34B5C4036840A27D93B13213E3324DD|thiV]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCTTTTACTTTCCAGCTTT, downstream forward: _UP4_AAGAAACGGAAGCGGGCGTC
  • BKK13230 (Δ[gene|9AF27E4CE34B5C4036840A27D93B13213E3324DD|thiV]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCTTTTACTTTCCAGCTTT, downstream forward: _UP4_AAGAAACGGAAGCGGGCGTC
  • References

  • 10092453,16291685,12376536,18763711,27447050