Save the date! 20th International Conference on Bacilli and Gram-Positive Bacteria. Washington, D.C. July 23-26, 2019.

yxaB

general stress protein, similar to pyruvyl transferase

locus: BSU_40030

pI: 4.53

mw: 39.61 kDa

protein length: 343 aa

gene length: 1032 bp

function: biofilm formation, survival of salt and ethanol stress

product: putative exopolysaccaride synthase

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.5|Other proteins required for biofilm formation]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 4,110,949 4,111,980

The protein

Protein family

• polysaccharide pyruvyl transferase family (with [protein|6EBD8F7065B6C1996067F3120DD9CFEA91D3A444|EpsI] and [protein|52A47B49B1FB955EE7E3C316B57A2B4134F78480|EpsO], according to UniProt)

Paralogous protein(s)

• [protein|6EBD8F7065B6C1996067F3120DD9CFEA91D3A444|EpsI]

Structure

• [PDB|5AX7] (from yeast, 30% identity) [pubmed|27194449]

Expression and Regulation

Operons

• genes: [gene|4907D4585491349E2592B0EF9150312A80785EE5|yxaA]-[gene|9A223AE85131511B7EA3A71EA57D87E8B167FA67|yxaB]
  description: [Pubmed|15805528,10746760]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|15805528], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|15805528,18326573], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|15805528]

• genes: [gene|9A223AE85131511B7EA3A71EA57D87E8B167FA67|yxaB]
  description: [Pubmed|15805528]

  regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|15805528]

Biological materials

Mutant

• MGNA-B681 (yxaB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1680 NBRP B. subtilis, Japan]
• BKE40030 ([gene|9A223AE85131511B7EA3A71EA57D87E8B167FA67|yxaB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE40030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCTGCGTCCTCCTTCT, downstream forward: _UP4_TAAACGAAAAAGACTGCCCT
• BKK40030 ([gene|9A223AE85131511B7EA3A71EA57D87E8B167FA67|yxaB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK40030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCTGCGTCCTCCTTCT, downstream forward: _UP4_TAAACGAAAAAGACTGCCCT

References

• 10746760,15805528,27194449