yfnG

similar to CDP-glucose 4,6-dehydratase

Locus: BSU_07280

Isoelectric point: 5.34

Molecular weight: 33.96 kDa

Protein length: 301 aa

Gene length: 906 bp

Function: unknown

Product: unknown

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other
• Groups of genes → Poorly characterized/ putative enzymes

Gene

Coordinates: 799,303 800,208

The protein

Protein family

• NAD(P)-dependent epimerase/dehydratase family (according to UniProt)

Paralogous protein(s)

• SpsJ
• YtcB:

Structure

• 4EGB (from Bacillus Anthracis Str.Ames, 31% identity)

Expression and Regulation

Operons

• Genes: yfnH-yfnG-yfnF-yfnE-yfnD
  Description: PubMed

  Sigma factors

  • SigK: sigma factor, PubMed, in SigK regulon
  • SigE: sigma factor, internal promoter in YfnE open reading frame PubMed, see DBTBS for details, in SigE regulon

  Regulatory mechanism

  • GerE: activation, PubMed, in GerE regulon

  Regulation

  • expressed during sporulation (SigK) PubMed

Biological materials

Mutant

• MGNA-C325 (yfnG::erm), available at the NBRP B. subtilis, Japan
• BKE07280 (yfnG::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ATTTTTCCAGAAACTCAAAT, downstream forward: _UP4_TAATGTGAAACGAGGTGAAC
• BKK07280 (yfnG::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ATTTTTCCAGAAACTCAAAT, downstream forward: _UP4_TAATGTGAAACGAGGTGAAC

References

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