thiT

S protein of thiamine [SW|ECF transporter]

locus: BSU_30990

pI: 10.24

mw: 20.31 kDa

protein length: 192 aa

gene length: 579 bp

function: thiamine uptake

product: S protein of thiamine [SW|ECF transporter]

essential: no

synonyms: yuaJ

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.3|ECF transporter]
• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.3|ECF transporter] → [category|SW 1.2.3.2|The substrate-specific S components of the ECF transporters]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.3|Biosynthesis/ acquisition of thiamine]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,179,306 3,179,884

The protein

Protein family

• vitamin uptake transporter (VUT/ECF) (TC 2.A.88) family (with [protein|97573C2D4FD8BE6B4C749C0644D053C9A0CBFFF3|TrpP] and [protein|7DD4BB8222B2EFE81C32C5521A29256095B9D6C1|YpdP], according to UniProt)

Structure

• [PDB|3RLB] (from ''Lactococcus lactis'', 32% identity) [Pubmed|21706007]

[SW|Localization]

• membrane [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|9685A380F95F1A73A693B53E4D35A1CB51043FEC|thiT]
  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box]: RNA switch, in [regulon|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box regulon]

  regulation

  • repressed by thiamine ([regulon|Thi-box|Thi-box]) [Pubmed|12376536]

Biological materials

Mutant

• MGNA-A222 (yuaJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/222 NBRP B. subtilis, Japan]
• BKE30990 ([gene|9685A380F95F1A73A693B53E4D35A1CB51043FEC|thiT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCTATCTCCTTCCATT, downstream forward: _UP4_TAAAAGTAACAATCCCCCAG
• BKK30990 ([gene|9685A380F95F1A73A693B53E4D35A1CB51043FEC|thiT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCTATCTCCTTCCATT, downstream forward: _UP4_TAAAAGTAACAATCCCCCAG

References

Reviews

• 20497229,22574898,24362466

Original publications

• 16291685,21706007,18763711,12376536,23602660