htpG

class III heat-shock protein (molecular chaperone)

locus: BSU_39820

pI: 4.67

mw: 72.08 kDa

protein length: 626 aa

gene length: 1881 bp

function: chaperone

product: class III heat-shock protein (molecular chaperone)

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.2|Chaperones/ protein folding]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]

Gene

Coordinates: 4,089,429 4,091,309

The protein

Protein family

• heat shock protein 90 family (single member, according to UniProt)

Structure

• [PDB|2IOQ] (from ''Escherichia coli'', 39% identity, 61% similarity) [Pubmed|17055434]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|962A955DED82EC0197757206A706796EC8F768BD|htpG]
  description: [Pubmed|9150201]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9150201], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulation

  • induced by high temperature [Pubmed|9150201]

Biological materials

Mutant

• GP1159 (del cat) available in [SW|Jörg Stülke]'s lab
• BKE39820 ([gene|962A955DED82EC0197757206A706796EC8F768BD|htpG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTCTTGATCGCTCCTTTA, downstream forward: _UP4_TAAACAGAAAAAGGAATCGT
• BKK39820 ([gene|962A955DED82EC0197757206A706796EC8F768BD|htpG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTCTTGATCGCTCCTTTA, downstream forward: _UP4_TAAACAGAAAAAGGAATCGT

References

• 9150201,12511492,10323241