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pdhA
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pdhA

pyruvate dehydrogenase (E1 alpha subunit), required for Z-ring assembly in a pyruvate-dependent manner
locus
BSU_14580
pI
5.84
mw
41.39 kDa
protein length
371 aa Sequence Blast
gene length
1116 bp Sequence Blast
function
links glycolysis and TCA cycle
product
pyruvate dehydrogenase (E1 alpha subunit)
essential
no
ec
1.2.4.1
synonyms
aceA

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.4|TCA cycle]
  • Gene

    Coordinates
    1,528,326 1,529,441

    Phenotypes of a mutant

  • ''pdhA'' is essential according to Kobayashi ''et al''. [Pubmed|12682299], non-essential according to [Pubmed|28189581]
  • the mutant grows slowly but is viable [Pubmed|24825009]
  • depletion of ''[gene|953DE0F0B81894ECFF4C0693511AC238BF3D0C0A|pdhA]'' and deletion of ''[gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA]'' have a strong synthetic defect in [SW|cell division] [Pubmed|24825009]
  • The protein

    Catalyzed reaction/ biological activity

  • [dihydrolipoyllysine-residue acetyltransferase]-(R)-N6-lipoyl-L-lysine + H+ + pyruvate --> [dihydrolipoyllysine-residue acetyltransferase]-(R)-N6-(S8-acetyldihydrolipoyl)-L-lysine + CO2 (according to UniProt)
  • Paralogous protein(s)

  • [protein|9F298088C0A9EB7FE140C935AFC9243C6D4DE8AE|BkdAA], [protein|B0A333BDFAE816B48B856A1DD91B73C41584A8E4|AcoA]
  • Kinetic information

  • Michaelis-Menten [Pubmed|6414463]
  • [SW|Cofactors]

  • thiamine pyrophosphate
  • Effectors of protein activity

  • Inhibited thiamine 2-thiothiazolone diphosphate and NADH [Pubmed|6414463]
  • Low sensibility to NADPH
  • Structure

  • [PDB|1W88] (E1 in complex with subunit binding domain of E2, ''Geobacillus stearothermophilus'')
  • [SW|Localization]

  • colocalizes with the nucleoid (depending on the availability of pyruvate) [Pubmed|24825009]
  • additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
  • Expression and Regulation

    Operons

    genes
    [gene|953DE0F0B81894ECFF4C0693511AC238BF3D0C0A|pdhA]-[gene|458E967052D1093A0F48AE0E6B6CCA0F52EAC44D|pdhB]-[gene|2F40086E35FA32136B9A89C530A86D714FE9460C|pdhC]-[gene|E9BBAE86DF3E536A987179CC394B472F6F710498|pdhD]
    description
    [Pubmed|11976308]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|20081037], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, due to presence of guanine at 1 position of the transcript [Pubmed|20081037], in [regulon|stringent response|stringent response]
  • regulation

  • ''[protein|search|pdhA]'': expression activated by glucose (1.9-fold) [Pubmed|12850135]
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    additional information

  • the mRNA belongs to the 46 most abundant mRNA species in dormant spores [pubmed|30782632]
  • Biological materials

    Mutant

  • BKE14580 ([gene|953DE0F0B81894ECFF4C0693511AC238BF3D0C0A|pdhA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACTAAGTCACCTCTTCC, downstream forward: _UP4_ACACAGAAGGAGTCGAAGTA
  • BKK14580 ([gene|953DE0F0B81894ECFF4C0693511AC238BF3D0C0A|pdhA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACTAAGTCACCTCTTCC, downstream forward: _UP4_ACACAGAAGGAGTCGAAGTA
  • lacZ fusion

  • pGP721 (in [SW|pAC5]), available in [SW|Jörg Stülke]'s lab, pGP186 (in [SW|pAC7]), available in [SW|Jörg Stülke]'s lab
  • labs

  • [SW|Arthur Aronson], Purdue University, West Lafayette, USA [http://wwwdev.gradschool.purdue.edu/PULSe/faculty.cfm?fid=5&range=0 homepage]
  • References

    Reviews

  • 19476487,9655937,2227213,6805383,24798336
  • Original publications

  • 9352926,20525796,12850135,6414463,11976308,20081037,15378759,24825009,28189581,28516784,30782632